AB126090

Anti-eRF3/GSPT1 antibody

Name: Anti-eRF3/GSPT1 antibody (ab126090) | Abcam
Brand: Abcam Limited
SKU: AB126090

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(9 Publications)

Anti-eRF3/GSPT1 antibody (ab126090) is a rabbit polyclonal antibody detecting eRF3/GSPT1 in Western Blot, IHC-P. Suitable for Human.

View Alternative Names

ERF3A, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF3/GSPT1 antibody (AB126090)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF3/GSPT1 antibody (AB126090)

ab126090 at 1/500 dilution, staining eRF3/GSPT1 in Paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

Supplier Data

Western blot - Anti-eRF3/GSPT1 antibody (AB126090)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-eRF3/GSPT1 antibody (ab126090) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-eRF3/GSPT1 antibody (ab126090) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of eRF3/GSPT1?
Anti-eRF3/GSPT1 (ab126090) specifically detects a band for eRF3/GSPT1 (UniProt: P15170) at a molecular weight of 69kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eRF3/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.

Biological function summary

GSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.

Pathways

GSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.

The role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed : 15987998, PubMed : 19417105, PubMed : 2511002, PubMed : 27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons : The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed : 16777602, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371).

See full target information GSPT1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 14:34 PubMed40885723

2025

Targeting pregnane X receptor with a potent agonist-based PROTAC to delay colon cancer relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Lucile Bansard,Guillaume Laconde,Vanessa Delfosse,Tiphaine Huet,Margaux Ayeul,Emilie Rigal,Quentin Donati,Sabine Gerbal-Chaloin,Martine Daujat-Chavanieu,Luc Brunel,Baptiste Legrand,Alain Chavanieu,Anthony R Martin,Julie Pannequin,William Bourguet,Muriel Amblard,Jean Marc Pascussi

Blood 142:629-642 PubMed37172201

2023

The orally bioavailable GSPT1/2 degrader SJ6986 exhibits in vivo efficacy in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yunchao Chang,Fatemeh Keramatnia,Pankaj S Ghate,Gisele Nishiguchi,Qingsong Gao,Ilaria Iacobucci,Lei Yang,Divyabharathi Chepyala,Ashutosh Mishra,Anthony A High,Hiroaki Goto,Koshi Akahane,Junmin Peng,Jun J Yang,Marcus Fischer,Zoran Rankovic,Charles G Mullighan

Journal of clinical laboratory analysis 37:e24810 PubMed36597856

2023

Hsa_circ_0001944 enhanced GSPT1 expression via sponging miR-498 to promote proliferation and invasion of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rujiao Liu,Xiaotian Han,Shuiping Gao,Yang Chen,Jian Zhang

Journal of medicinal chemistry 64:7296-7311 PubMed34042448

2021

Identification of Potent, Selective, and Orally Bioavailable Small-Molecule GSPT1/2 Degraders from a Focused Library of Cereblon Modulators.

Applications

Unspecified application

Species

Unspecified reactive species

Gisele Nishiguchi,Fatemeh Keramatnia,Jaeki Min,Yunchao Chang,Barbara Jonchere,Sourav Das,Marisa Actis,Jeanine Price,Divyabharathi Chepyala,Brandon Young,Kevin McGowan,P Jake Slavish,Anand Mayasundari,Jamie A Jarusiewicz,Lei Yang,Yong Li,Xiang Fu,Shalandus H Garrett,James B Papizan,Kiran Kodali,Junmin Peng,Shondra M Pruett Miller,Martine F Roussel,Charles Mullighan,Marcus Fischer,Zoran Rankovic

Journal of medicinal chemistry 63:15883-15905 PubMed33284613

2020

Potent and Selective Mitogen-Activated Protein Kinase Kinase 1/2 (MEK1/2) Heterobifunctional Small-molecule Degraders.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Hu,Jieli Wei,Hyerin Yim,Li Wang,Ling Xie,Margaret S Jin,Md Kabir,Lihuai Qin,Xian Chen,Jing Liu,Jian Jin

Journal of Cancer 11:2201-2212 PubMed32127947

2020

Lnc-SNHG16/miR-128 axis modulates malignant phenotype through WNT/β-catenin pathway in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wu Wu,Li Guo,Zhenlong Liang,Yuanbin Liu,Zhi Yao

Journal of medicinal chemistry 62:9471-9487 PubMed31560543

2019

Simple Structural Modifications Converting a Bona fide MDM2 PROTAC Degrader into a Molecular Glue Molecule: A Cautionary Tale in the Design of PROTAC Degraders.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuling Yang,Yangbing Li,Angelo Aguilar,Zhaomin Liu,Chao-Yie Yang,Shaomeng Wang

OncoTargets and therapy 12:3945-3954 PubMed31190891

2019

LncRNA promotes the proliferation, invasion, and migration of non-small cell lung cancer cells by targeting the / axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Sun,Liwen Zhang,Ranran Yan,Ying Yang,Xiangli Meng

Journal of medicinal chemistry 61:6685-6704 PubMed30019901

2018

Discovery of QCA570 as an Exceptionally Potent and Efficacious Proteolysis Targeting Chimera (PROTAC) Degrader of the Bromodomain and Extra-Terminal (BET) Proteins Capable of Inducing Complete and Durable Tumor Regression.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Qin,Yang Hu,Bing Zhou,Ester Fernandez-Salas,Chao-Yie Yang,Liu Liu,Donna McEachern,Sally Przybranowski,Mi Wang,Jeanne Stuckey,Jennifer Meagher,Longchuan Bai,Zhuo Chen,Mei Lin,Jiuling Yang,Danya N Ziazadeh,Fuming Xu,Jiantao Hu,Weiguo Xiang,Liyue Huang,Siwei Li,Bo Wen,Duxin Sun,Shaomeng Wang

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-eRF3/GSPT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF3/GSPT1 antibody (AB126090)

Western blot - Anti-eRF3/GSPT1 antibody (AB126090)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise