Anti-eRF3/GSPT1 antibody (ab126090) is a rabbit polyclonal antibody detecting eRF3/GSPT1 in Western Blot, IHC-P. Suitable for Human.
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- Journal of clinical laboratory analysis 37:e248102023Hsa_circ_0001944 enhanced GSPT1 expression via sponging miR-498 to promote proliferation and invasion of gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRujiao Liu,Xiaotian Han,Shuiping Gao,Yang Chen,Jian ZhangPubMed 36597856
- Journal of medicinal chemistry 64:7296-73112021Identification of Potent, Selective, and Orally Bioavailable Small-Molecule GSPT1/2 Degraders from a Focused Library of Cereblon Modulators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGisele Nishiguchi,Fatemeh Keramatnia,Jaeki Min,Yunchao Chang,Barbara Jonchere,Sourav Das,Marisa Actis,Jeanine Price,Divyabharathi Chepyala,Brandon Young,Kevin McGowan,P Jake Slavish,Anand Mayasundari,Jamie A Jarusiewicz,Lei Yang,Yong Li,Xiang Fu,Shalandus H Garrett,James B Papizan,Kiran Kodali,Junmin Peng,Shondra M Pruett Miller,Martine F Roussel,Charles Mullighan,Marcus Fischer,Zoran RankovicPubMed 34042448
- Journal of medicinal chemistry 63:15883-159052020Potent and Selective Mitogen-Activated Protein Kinase Kinase 1/2 (MEK1/2) Heterobifunctional Small-molecule Degraders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianping Hu,Jieli Wei,Hyerin Yim,Li Wang,Ling Xie,Margaret S Jin,Md Kabir,Lihuai Qin,Xian Chen,Jing Liu,Jian JinPubMed 33284613
- Journal of Cancer 11:2201-22122020Lnc-SNHG16/miR-128 axis modulates malignant phenotype through WNT/β-catenin pathway in cervical cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWu Wu et. al.PubMed 32127947
- Journal of medicinal chemistry 62:9471-94872019Simple Structural Modifications Converting a Bona fide MDM2 PROTAC Degrader into a Molecular Glue Molecule: A Cautionary Tale in the Design of PROTAC Degraders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiuling Yang et. al.PubMed 31560543
- OncoTargets and therapy 12:3945-39542019LncRNA promotes the proliferation, invasion, and migration of non-small cell lung cancer cells by targeting the / axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Sun et. al.PubMed 31190891
- Journal of medicinal chemistry 61:6685-67042018Discovery of QCA570 as an Exceptionally Potent and Efficacious Proteolysis Targeting Chimera (PROTAC) Degrader of the Bromodomain and Extra-Terminal (BET) Proteins Capable of Inducing Complete and Durable Tumor Regression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Qin et. al.PubMed 30019901
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
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Target data
Function
GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:15987998, PubMed:19417105, PubMed:2511002, PubMed:27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed:16777602, PubMed:27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Alternative names
ERF3A, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog
Recommended products
Anti-eRF3/GSPT1 antibody (ab126090) is a rabbit polyclonal antibody detecting eRF3/GSPT1 in Western Blot, IHC-P. Suitable for Human.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-eRF3/GSPT1 antibody (ab126090) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of eRF3/GSPT1?
Anti-eRF3/GSPT1 (ab126090) specifically detects a band for eRF3/GSPT1 (UniProt: P15170) at a molecular weight of 69kDa.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eRF3/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.
- Biological function summary
GSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.
- Pathways
GSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.
- Associated diseases and disorders
The role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-eRF3/GSPT1 antibody (ab126090)
All lanes: Western blot - Anti-eRF3/GSPT1 antibody (ab126090) at 1/10000 dilution
Lane 1: 293T cells
Lane 2: A431 cells
Lane 3: HeLa cells
Lane 4: HepG2 cells
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF3/GSPT1 antibody (ab126090)
ab126090 at 1/500 dilution, staining eRF3/GSPT1 in Paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
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