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AB234433

Anti-eRF3/GSPT1 antibody [EPR22908-103]

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(1 Publication)

Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) is a rabbit monoclonal antibody detecting eRF3/GSPT1 in Western Blot. Suitable for Human, Mouse,.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

ERF3A, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog

2 Images
Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (AB234433)
  • WB

Lab

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (AB234433)

This antibody recognizes unidentifiable weak bands below 50kDa

Exposure time : Lanes 1-2 : 3 seconds Lanes 3-4 : 37 seconds

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 80 kDa

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Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (AB234433)
  • WB

Lab

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (AB234433)

Exposure time : 15 seconds

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

Lane 1:

His tagged human eRF3a recombinant protein, 10 ng

Lane 2:

His tagged human eRF3b recombinant protein, 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 48 kDa

false

  • Carrier free

    Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22908-103

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR22908-103] also available for your convenience: ab234433, Carrier free - ab256478

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eRF3/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.
Biological function summary

GSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.

Pathways

GSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.

The role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed : 15987998, PubMed : 19417105, PubMed : 2511002, PubMed : 27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons : The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed : 16777602, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371).
See full target information GSPT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:1958-1971 PubMed36692137

2023

Crizotinib-based proteolysis targeting chimera suppresses gastric cancer by promoting MET degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Jiao Chen,Jin-Mei Jin,Wen-Jie Gu,Zeng Zhao,Hu Yuan,Yu-Dong Zhou,Dale G Nagle,Qiu-Lei Xi,Xue-Mei Zhang,Qing-Yan Sun,Ye Wu,Wei-Dong Zhang,Xin Luan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB126090

Anti-eRF3/GSPT1 antibody

primary-antibodies

erf3-gspt1-antibody-ab126090

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