AB256478

Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal eRF3/GSPT1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.

View Alternative Names

ERF3A, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog

2 Images

Lab

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (AB256478)

This antibody recognizes unidentifiable weak bands below 50kDa

Exposure time : Lanes 1-2 : 3 seconds Lanes 3-4 : 37 seconds

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234433).

All lanes:

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (<a href='/en-us/products/primary-antibodies/erf3-gspt1-antibody-epr22908-103-ab234433'>ab234433</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 80 kDa

false

Lab

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (AB256478)

Exposure time : 15 seconds

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234433).

All lanes:

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (<a href='/en-us/products/primary-antibodies/erf3-gspt1-antibody-epr22908-103-ab234433'>ab234433</a>) at 1/1000 dilution

Lane 1:

His tagged human eRF3a recombinant protein, 10 ng

Lane 2:

His tagged human eRF3b recombinant protein, 10ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 56 kDa

Observed band size: 48 kDa

false

Unconjugated

Anti-eRF3/GSPT1 antibody [EPR22908-103]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22908-103

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab256478 is the carrier-free version of ab234433.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eRF3/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.

Biological function summary

GSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.

Pathways

GSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.

The role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed : 15987998, PubMed : 19417105, PubMed : 2511002, PubMed : 27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons : The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed : 27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed : 16777602, PubMed : 27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed : 24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed : 30682371).

See full target information GSPT1

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (AB256478)

Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (AB256478)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise