Rabbit Recombinant Monoclonal eRF3/GSPT1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407957.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525)")
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407956.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525)")
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407958.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525)")
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407955.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525)")
![Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--immunoprecipitation-img407954.png/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (AB303525)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Expected |
| Rat | Tested | Not recommended | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:15987998, PubMed:19417105, PubMed:2511002, PubMed:27863242). GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:27863242). GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed:16777602, PubMed:27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371).
Alternative names
ERF3A, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homolog
Recommended products
Rabbit Recombinant Monoclonal eRF3/GSPT1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27296-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eRF3/GSPT1 protein also known as GSPT1 plays an important role in translation termination. It works with eRF1 to release the newly synthesized polypeptide chain from the ribosome. GSPT1 is a GTPase of approximately 69 kDa commonly expressed in human tissues with higher concentrations found in proliferating cells such as those in the bone marrow and lymphoid tissues. It implements essential tasks in protein synthesis regulation by facilitating proper translation termination.
- Biological function summary
GSPT1 is involved in processes beyond translation termination. It associates with complexes that regulate mRNA translation and stability. As part of the eRF3 pathway GSPT1 interacts with various translation and post-translation machinery. Its molecular function extends to include potential interactions with lncRNAs such as lnc-SNHG16 indicating a role in cellular processes like cell growth and division. GSPT1's interaction network suggests that it performs multifunction in cell regulation.
- Pathways
GSPT1 integrates into the translation termination pathway tightly linking with the mRNA surveillance pathway. This protein is essential for efficient recognition and release of stop codons. In these pathways it collaborates with proteins such as eRF1 to regulate mRNA stability and translation accuracy. These interactions ensure the fidelity of protein biosynthesis and proper cellular function.
- Associated diseases and disorders
The role of GSPT1 extends to cancer and neurodegenerative diseases. Overexpression or mutations of GSPT1 links to various cancers including gastric and colorectal cancers where it affects cell proliferation pathways. The GSPT1-Myc axis appears to contribute significantly to oncogenesis acting with proteins like c-Myc to promote tumorigenesis. Additionally disruptions in GSPT1 function could associate with neurological disorders where translation termination processes malfunction although these aspects require further study.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407957.png "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] (Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524) at 1/1000 dilution
All lanes: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 80 kDa
Exposure time: 3min
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407956.png "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Lysates were freshly made and used immediately to minimize protein degradation.
All lanes: Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] (Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Observed band size: 80 kDa
Exposure time: 3min
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407958.png "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBSTAll lanes: Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] (Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524) at 1/1000 dilution
Lane 1: HEK-293T transfected with human ERF3A expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with human ERF3B expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 80 kDa
Exposure time: 5.5s
![Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--western-blot-img407955.png "Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 136 seconds
Lane 2-5: 48 seconds
In lane 1, the lysates were stored at -80℃ prior to Western Blotting.
The bands beneath the target band (80 kDa) are expected to be degradation products.
In lane 2-5, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.All lanes: Western blot - Anti-eRF3A/GSPT1 antibody [EPR27296-4] (Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524) at 1/1000 dilution
Lanes 1 - 2: HeLa (human epithelial cell line from cervical adenocarcinoma), whole cell lysate at 20 µg
Lane 3: 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 80 kDa
![Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--immunoprecipitation-img407954.png "Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
eRF3A/GSPT1 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate 10 ug with Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate 10 µg (Inset)
Lane 2: Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 IP in PC-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 in PC-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.All lanes: Immunoprecipitation - Anti-eRF3A/GSPT1 antibody [EPR27296-4] (Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524) at 1/30 dilution
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) at 10 µg
Lane 2: Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 IP in PC-3 whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 56 kDa
Observed band size: 80 kDa
Exposure time: 3s
![Flow Cytometry - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--flow-cytometry-img402344.jpg "Flow Cytometry - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Flow Cytometry - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling eRF3A/GSPT1 with Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--immunocytochemistry-immunofluorescence-img402315.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
Immunocytochemistry/ Immunofluorescence - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)
This data was developed using Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling eRF3A/GSPT1 with Anti-eRF3A/GSPT1 antibody [EPR27296-4] ab303524 at 1/100 (4.94 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly cytoplasmic staining in PC-3 cell lines. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Flow Cytometry - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--flow-cytometry-img402344.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
![Immunocytochemistry/ Immunofluorescence - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525), expandable thumbnail](https://content.abcam.com/products/images/erf3a-gspt1-antibody-epr27296-4-bsa-and-azide-free-ab303525--immunocytochemistry-immunofluorescence-img402315.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eRF3A/GSPT1 antibody [EPR27296-4] - BSA and Azide free (ab303525)")
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