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AB96697

Anti-ERG antibody

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(1 Publication)

Rabbit Polyclonal ERG antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERG aa 400 to C-terminus.

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (AB96697)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (AB96697)

Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
Lower image : merged with DNA probe.

Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (AB96697)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (AB96697)

Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.

Western blot - Anti-ERG antibody (AB96697)
  • WB

Unknown

Western blot - Anti-ERG antibody (AB96697)

10% SDS PAGE

All lanes:

Western blot - Anti-ERG antibody (ab96697) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 55 kDa

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Western blot - Anti-ERG antibody (AB96697)
  • WB

Supplier Data

Western blot - Anti-ERG antibody (AB96697)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERG antibody (ab96697) at 1/10000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ERG aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11308

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.
Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
See full target information ERG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 4:1623 PubMed23535644

2013

Monoallelic expression of TMPRSS2/ERG in prostate cancer stem cells.

Applications

Unspecified application

Species

Human

Euan S Polson,John L Lewis,Hamza Celik,Vincent M Mann,Michael J Stower,Matthew S Simms,Greta Rodrigues,Anne T Collins,Norman J Maitland
View all publications
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