Rabbit Polyclonal ERG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERG aa 400 to C-terminus.
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- Nature communications 4:16232013Monoallelic expression of TMPRSS2/ERG in prostate cancer stem cells.Applications:Unspecified applicationReactive species:HumanEuan S Polson,John L Lewis,Hamza Celik,Vincent M Mann,Michael J Stower,Matthew S Simms,Greta Rodrigues,Anne T Collins,Norman J MaitlandPubMed 23535644
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ERG aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11308
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Alternative names
Transcriptional regulator ERG, Transforming protein ERG, ERG
Recommended products
Rabbit Polyclonal ERG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERG aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ERG aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11308
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.
- Biological function summary
ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.
- Pathways
ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.
- Associated diseases and disorders
ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ERG antibody (ab96697)
10% SDS PAGE
All lanes: Western blot - Anti-ERG antibody (ab96697) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (ab96697)
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
Lower image: merged with DNA probe.
Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody (ab96697)
Immunofluorescence analysis of ERG in paraformaldehyde fixed Human pancreatic cancer cell line, using ab96697 at a 1/50 dilution.
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