AB110639

Anti-ERG antibody [EPR3863]

Name: Anti-ERG antibody [EPR3863] (ab110639) | Abcam
Brand: Abcam Limited
SKU: AB110639
Price: 375 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(2 Reviews)

(31 Publications)

Anti-ERG antibody [EPR3863] (ab110639) is a rabbit monoclonal antibody detecting ERG in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (AB110639)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (AB110639)

ab110639 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (AB110639)

ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ERG antibody [EPR3863] (AB110639)

All lanes:

Western blot - Anti-ERG antibody [EPR3863] (ab110639) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HT29 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Predicted band size: 54 kDa

false

Carrier free

Anti-ERG antibody [EPR3863] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ERG antibody [EPR3863]
660 APC

APC Anti-ERG antibody [EPR3863]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ERG antibody [EPR3863]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ERG antibody [EPR3863]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ERG antibody [EPR3863]
578 PE

PE Anti-ERG antibody [EPR3863]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-ERG antibody [EPR3863]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3863

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ERG antibody [EPR3863] (ab110639) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of ERG?
Anti-ERG [EPR3863] (ab110639) specifically detects a band for ERG (UniProt: P11308) at a molecular weight of 55kDa.

Trusted by the scientific community
Anti-ERG [EPR3863] (ab110639) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3863] also available for your convenience: ab110639, Carrier free - ab239989, APC - ab319244, PE - ab319392, Alexa Fluor® 488 - ab319526, Alexa Fluor® 647 - ab319639, Alexa Fluor® 594 - ab319743, Alexa Fluor® 555 - ab319885, Alexa Fluor® 750 - ab320965

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.

Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

See full target information ERG

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:808455 PubMed35004698

2021

CCRL2 Modulates Physiological and Pathological Angiogenesis During Retinal Development.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Ben Dhaou,Annalisa Del Prete,Silvano Sozzani,Marc Parmentier

Angiogenesis 25:159-179 PubMed34524600

2021

Chemerin regulates normal angiogenesis and hypoxia-driven neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Ben Dhaou,Kamel Mandi,Mickaël Frye,Angela Acheampong,Ayoub Radi,Benjamin De Becker,Mathieu Antoine,Nicolas Baeyens,Valérie Wittamer,Marc Parmentier

Nature 594:271-276 PubMed33910229

2021

PIK3CA and CCM mutations fuel cavernomas through a cancer-like mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Aileen A Ren,Daniel A Snellings,Yourong S Su,Courtney C Hong,Marco Castro,Alan T Tang,Matthew R Detter,Nicholas Hobson,Romuald Girard,Sharbel Romanos,Rhonda Lightle,Thomas Moore,Robert Shenkar,Christian Benavides,M Makenzie Beaman,Helge Müller-Fielitz,Mei Chen,Patricia Mericko,Jisheng Yang,Derek C Sung,Michael T Lawton,J Michael Ruppert,Markus Schwaninger,Jakob Körbelin,Michael Potente,Issam A Awad,Douglas A Marchuk,Mark L Kahn

Cancer research 81:3766-3776 PubMed33632899

2021

Susceptibility-Associated Genetic Variation in Contributes to Prostate Cancer Initiation and Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Han,Jude N Owiredu,Bridget M Healy,Muqing Li,Maryam Labaf,Jocelyn S Steinfeld,Susan Patalano,Shuai Gao,Mingyu Liu,Jill A Macoska,Kourosh Zarringhalam,Kellee R Siegfried,Xin Yuan,Timothy R Rebbeck,Changmeng Cai

EMBO reports 22:e48961 PubMed33512764

2021

EVL regulates VEGF receptor-2 internalization and signaling in developmental angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Joana Zink,Maike Frye,Timo Frömel,Claudia Carlantoni,David John,Danny Schreier,Andreas Weigert,Hebatullah Laban,Gabriela Salinas,Heike Stingl,Lea Günther,Rüdiger Popp,Jiong Hu,Benoit Vanhollebeke,Hannes Schmidt,Amparo Acker-Palmer,Thomas Renné,Ingrid Fleming,Peter M Benz

Developmental biology 473:1-14 PubMed33453264

2021

ETS factors are required but not sufficient for specific patterns of enhancer activity in different endothelial subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Neal,Svanhild Nornes,Pakavarin Louphrasitthiphol,Natalia Sacilotto,Mark D Preston,Lucija Fleisinger,Sophie Payne,Sarah De Val

The Journal of clinical investigation 130:4235-4251 PubMed32427589

2020

Blocking endothelial apoptosis revascularizes the retina in a model of ischemic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zoe L Grant,Lachlan Whitehead,Vickie Hy Wong,Zheng He,Richard Y Yan,Abigail R Miles,Andrew V Benest,David O Bates,Claudia Prahst,Katie Bentley,Bang V Bui,Robert Ca Symons,Leigh Coultas

Development (Cambridge, England) 147: PubMed32341028

2020

Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Koth,Xiaonan Wang,Abigail C Killen,William T Stockdale,Helen G Potts,Andrew Jefferson,Florian Bonkhofer,Paul R Riley,Roger K Patient,Berthold Göttgens,Mathilda T M Mommersteeg

Biomolecules 10: PubMed32210087

2020

BMP-SMAD1/5 Signaling Regulates Retinal Vascular Development.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Benn,Florian Alonso,Jo Mangelschots,Elisabeth Génot,Marleen Lox,An Zwijsen

eLife 8: PubMed31789590

2019

Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Stanislao Igor Travisano,Vera Lucia Oliveira,Belén Prados,Joaquim Grego-Bessa,Rebeca Piñeiro-Sabarís,Vanesa Bou,Manuel J Gómez,Fátima Sánchez-Cabo,Donal MacGrogan,José Luis de la Pompa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERG antibody [EPR3863]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (AB110639)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (AB110639)

Western blot - Anti-ERG antibody [EPR3863] (AB110639)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (31)

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