AB239989

Anti-ERG antibody [EPR3863] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ERG antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] - BSA and Azide free (AB239989)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] - BSA and Azide free (AB239989)

ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110639).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unconjugated

Anti-ERG antibody [EPR3863]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-ERG antibody [EPR3863]
578 PE

PE Anti-ERG antibody [EPR3863]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-ERG antibody [EPR3863]
660 APC

APC Anti-ERG antibody [EPR3863]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-ERG antibody [EPR3863]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ERG antibody [EPR3863]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ERG antibody [EPR3863]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3863

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab239989 is the carrier-free version of ab110639.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.

Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

See full target information ERG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:554831 PubMed34336814

2021

Transcription Factor DLX5 Promotes Hair Follicle Stem Cell Differentiation by Regulating the c-MYC/microRNA-29c-3p/NSD1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bojie Lin,Jiangying Zhu,Guoqian Yin,Mingde Liao,Guanyu Lin,Yuyong Yan,Dan Huang,Siding Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERG antibody [EPR3863] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] - BSA and Azide free (AB239989)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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