AB170994

Anti-ERG2 antibody [EPR11840]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ERG2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ERG2, KCNH6, Voltage-gated inwardly rectifying potassium channel KCNH6, Ether-a-go-go-related gene potassium channel 2, Potassium voltage-gated channel subfamily H member 6, Voltage-gated potassium channel subunit Kv11.2, ERG-2, Eag-related protein 2, Ether-a-go-go-related protein 2, hERG-2, hERG2

1 Images

Supplier Data

Western blot - Anti-ERG2 antibody [EPR11840] (AB170994)

Primary antibody diluted in 1% BSA.

All lanes:

Western blot - Anti-ERG2 antibody [EPR11840] (ab170994) at 1/1000 dilution

Lane 1:

SH-SY5Y lysate at 10 µg

Lane 2:

Y79 lysate at 10 µg

Lane 3:

HT-1080 lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Lane 5:

MDA-MB-435 lysate at 10 µg

Predicted band size: 110 kDa

false

Carrier free

Anti-ERG2 antibody [EPR11840] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11840

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG2 also known as sterol isomerase or C-8 sterol isomerase is an enzyme in the ergosterol biosynthesis pathway important for sterol production. This protein has a mass of approximately 25 kDa. ERG2 is found mainly in the endoplasmic reticulum and is highly expressed in yeast and fungi. ERG2's primary role involves catalyzing the isomerization of sterols an important step in ergosterol synthesis from which the eukaryotic cell membranes gain structural integrity.

Biological function summary

Sterol isomerase activity is significant in maintaining membrane fluidity and sterol homeostasis. ERG2 functions as part of the larger ergosterol biosynthesis enzyme complex. The integrity and function of the cellular membrane depend on the proper functioning of this complex with ERG2 being one of the essential modulators within it.

Pathways

ERG2 operates within the ergosterol biosynthesis pathway which is part of a larger lipid metabolic pathway important for maintaining cellular functions. This pathway includes several other enzymes like ERG3 and ERG6 which work consecutively with ERG2. Disruptions in this pathway can lead to cell membrane instability and defects in organismal growth regulation.

Defects or inhibition of ERG2 can result in problems like ergosterol biosynthesis disorders primarily in fungi making it an important target for antifungal drugs. Conditions related to ERG2 also involve cholesterol synthesis in broader biological systems affected through its interactions with proteins like ERG11. Disorders arising from ergosterol pathway disruptions may affect cellular proliferation and development leading to broader systemic implications in pathogenic fungi.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Characterized by unusual gating kinetics by producing relatively small outward currents during membrane depolarization and large inward currents during subsequent repolarization which reflect a rapid inactivation during depolarization and quick recovery from inactivation but slow deactivation (closing) during repolarization. Activates even more slowly than KCNH2.

See full target information KCNH6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 8:92 PubMed35853899

2022

LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kedariti,Emanuele Frattini,Pascale Baden,Susanna Cogo,Laura Civiero,Elena Ziviani,Gianluca Zilio,Federico Bertoli,Massimo Aureli,Alice Kaganovich,Mark R Cookson,Leonidas Stefanis,Matthew Surface,Michela Deleidi,Alessio Di Fonzo,Roy N Alcalay,Hardy Rideout,Elisa Greggio,Nicoletta Plotegher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERG2 antibody [EPR11840]

Western blot - Anti-ERG2 antibody [EPR11840] (AB170994)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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