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AB121582

Anti-ERGIC1 antibody

2

(1 Review)

|

(2 Publications)

Rabbit Polyclonal ERGIC1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERGIC1 aa 100-200.

View Alternative Names

ERGIC32, KIAA1181, HT034, ERGIC1, Endoplasmic reticulum-Golgi intermediate compartment protein 1, ER-Golgi intermediate compartment 32 kDa protein, ERGIC-32

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (AB121582)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (AB121582)

Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (AB121582)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (AB121582)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (AB121582)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (AB121582)

ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.

Western blot - Anti-ERGIC1 antibody (AB121582)
  • WB

Unknown

Western blot - Anti-ERGIC1 antibody (AB121582)

All lanes:

Western blot - Anti-ERGIC1 antibody (ab121582) at 1/250 dilution

Lane 1:

Vector only transfected HEK293T cell lysate

Lane 2:

Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 33 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ERGIC1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q969X5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p>Use buffer at pH 6 for antigen retrieval.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ER-Golgi Intermediate Compartment 1 (ERGIC1) is an important protein that functions mechanically in protein sorting and trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus. Known also as ERGIC-53 ERGIC-1 is a significant component involved in shuttling cargo proteins ensuring proper delivery through secretory pathways. It weighs approximately 59 kDa and is expressed in various cell types found extensively in the ER-Golgi interfaces.
Biological function summary

The movement processes mediated by ERGIC-1 maintain the central operations of intracellular transport. It plays an important role in the cycling and movement of glycoproteins influencing different cellular activities. ERGIC1 is part of a larger complex involved in vesicular transport and interacts with several coat protein complexes. These activities are vital for the modulation of glycoprotein processing and trafficking linking its function intricately with cellular transport mechanisms.

Pathways

Researchers have linked ERGIC-1 to important activities like the secretory pathway and protein synthesis pathways. Within these pathways it coordinates with proteins such as COPI and COPII ensuring efficient vesicle formation and cargo delivery between the ER and Golgi. Efficient functioning of these pathways is necessary for the regulation of protein synthesis and secretion directly impacting cellular homeostasis.

ERGIC-1 plays a role in conditions affecting protein transport and folding. Misregulation or mutations in ERGIC1 can lead to disorders such as congenital disorders of glycosylation. Additionally researchers observe that it influences pathways involving proteins like lysosomal alpha-glucosidase connecting it to diseases like Pompe disease. Understanding these connections highlights ERGIC-1's importance in maintaining cellular function and health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possible role in transport between endoplasmic reticulum and Golgi.
See full target information ERGIC1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The American journal of the medical sciences 355:228-234 PubMed29549924

2018

Differential Expression and Significance of Endoplasmic Reticulum Golgi Intermediate Compartment 1 in Precancerous Gastric Lesions and Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Furong Wang,Xiaoying Guan,Jinwei Yang,Wenting He,Yucai Wei,Hao Chen,Yumin Li

World journal of gastroenterology 23:6119-6127 PubMed28970727

2017

Aberrant DNA-PKcs and ERGIC1 expression may be involved in initiation of gastric cancer.

Applications

ICC/IF

Species

Unspecified reactive species

Fu-Rong Wang,Yu-Cai Wei,Zhi-Jian Han,Wen-Ting He,Xiao-Ying Guan,Hao Chen,Yu-Min Li
View all publications
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Product promise

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