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AB237761

Anti-ERGIC2/PTX1 antibody

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(2 Publications)

Rabbit Polyclonal ERGIC2/PTX1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERGIC2 aa 50-350.

View Alternative Names

ERV41, PTX1, CDA14, ERGIC2, Endoplasmic reticulum-Golgi intermediate compartment protein 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC2/PTX1 antibody (AB237761)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC2/PTX1 antibody (AB237761)

Paraffin-embedded human lung cancer tissue stained for ERGIC2/PTX1 with ab237761 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC2/PTX1 antibody (AB237761)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC2/PTX1 antibody (AB237761)

Paraffin-embedded human pancreas tissue stained for ERGIC2/PTX1 with ab237761 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC2/PTX1 antibody (AB237761)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC2/PTX1 antibody (AB237761)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for ERGIC2/PTX1 using ab237761 at 1/100 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Western blot - Anti-ERGIC2/PTX1 antibody (AB237761)
  • WB

Supplier Data

Western blot - Anti-ERGIC2/PTX1 antibody (AB237761)

All lanes:

Western blot - Anti-ERGIC2/PTX1 antibody (ab237761) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Rat brain tissue lysate

Lane 3:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ERGIC2 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q96RQ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERGIC2 also known as PTX1 is involved in transport and processing within the endoplasmic reticulum-Golgi intermediate compartment. ERGIC2 has a mass of approximately 45 kDa. It is an integral membrane protein widely expressed in various tissues and particularly prominent in the liver and kidney. This protein plays a significant role in regulating the trafficking of proteins between the endoplasmic reticulum and the Golgi apparatus important for proper cellular function.
Biological function summary

ERGIC2 contributes to the maintenance of the cellular infrastructure by facilitating the efficient transport of proteins. It is an essential component of the early secretory pathway and plays a role in protein sorting and vesicle formation. ERGIC2 participates in a well-defined protein complex associated with COPII-coated vesicles which aid in cargo transport from the endoplasmic reticulum to the Golgi. Its interaction within this complex highlights its importance in cellular logistics and management.

Pathways

ERGIC2 participates actively in the ER-Golgi transport pathway and the secretory pathway. These pathways are essential for efficient protein trafficking and processing. ERGIC2 interacts with other proteins such as COPII components including SEC23 and SEC24 which are integral to the formation of transport vesicles. These interactions highlight how ERGIC2 fits into larger pathways that ensure cellular homeostasis and functionality.

Alterations in ERGIC2 expression have been observed in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Insufficient or excess ERGIC2 can impair protein trafficking leading to cellular stress and disease progression. Its role in these disorders bridges it to proteins like APP (Amyloid precursor protein) in Alzheimer's disease potentially affecting amyloid processing and aggregation. Understanding ERGIC2's function may offer insights into therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possible role in transport between endoplasmic reticulum and Golgi.
See full target information ERGIC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 22:e14009 PubMed37960952

2023

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zer Vue,Edgar Garza-Lopez,Kit Neikirk,Prasanna Katti,Larry Vang,Heather Beasley,Jianqiang Shao,Andrea G Marshall,Amber Crabtree,Alexandria C Murphy,Brenita C Jenkins,Praveena Prasad,Chantell Evans,Brittany Taylor,Margaret Mungai,Mason Killion,Dominique Stephens,Trace A Christensen,Jacob Lam,Benjamin Rodriguez,Mark A Phillips,Nastaran Daneshgar,Ho-Jin Koh,Alice Koh,Jamaine Davis,Nina Devine,Mohammad Saleem,Estevão Scudese,Kenneth Ryan Arnold,Valeria Vanessa Chavarin,Ryan Daniel Robinson,Moumita Chakraborty,Jennifer A Gaddy,Mariya T Sweetwyne,Genesis Wilson,Elma Zaganjor,James Kezos,Cristiana Dondi,Anilkumar K Reddy,Brian Glancy,Annet Kirabo,Anita M Quintana,Dao-Fu Dai,Karen Ocorr,Sandra A Murray,Steven M Damo,Vernat Exil,Blake Riggs,Bret C Mobley,Jose A Gomez,Melanie R McReynolds,Antentor Hinton

International journal of cardiology 315:72-80 PubMed32349937

2020

LncRNA ZFAS1 confers inflammatory responses and reduces cholesterol efflux in atherosclerosis through regulating miR-654-3p-ADAM10/RAB22A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Tang,Ruohan Yin,Haifeng Shi,Xiang Wang,Dong Shen,Xiaoqin Wang,Changjie Pan
View all publications
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Product promise

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