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Rabbit Recombinant Monoclonal ERGIC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.


Images

Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (AB129179), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (AB129179), expandable thumbnail
  • Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179), expandable thumbnail
  • OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (AB129179), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ERGIC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8141
Purification technique
Affinity purification Protein A
Dissociation constant
5.36 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ERGIC3 protein also known as ERGIC-53 plays a critical role in the early secretory pathway. It is involved in vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus. Weighing around 38 kDa ERGIC3 is an important contributor to cargo transport processes. The protein finds expression in various tissues predominantly within cells that have high secretory demands.

Biological function summary

The ERGIC3 protein functions as part of the larger ER-Golgi intermediate compartment (ERGIC) complex helping in the sorting and transport of proteins. The ERGIC serves as an essential hub in the secretory pathway modulating cargo movement to the Golgi for further processing. Some studies have implicated ERGIC3 in maintaining Golgi integrity and optimal function affecting overall cellular health.

Pathways

ERGIC3 plays a significant role in the secretory pathway assisting in the transit of proteins and the correct folding of polypeptides. It links functionally to other key proteins such as KI67 which associates with cell proliferation regions and relies heavily on efficient protein processing for cellular division. The association suggests ERGIC3’s involvement in maintaining cellular growth and regeneration.

Associated diseases and disorders

Researchers have connected ERGIC3 with diseases like congenital disorders of glycosylation due to its role in protein folding and trafficking. Furthermore disruptions in ERGIC3 function have correlations with cancer progression particularly in overactive secretion conditions. These disorders often coincide with aberrant expression or mutations in folding-related proteins signifying potential ERGIC3 involvement in pathological manifestations when its function is compromised.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179), expandable thumbnail

    Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    Lanes 1-4: Merged signal (red and green). Green - ab129179 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ERGIC3 knockout HEK-293T cell line ab266489 (knockout cell lysate Human ERGIC3 knockout HEK-293T cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ERGIC3 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human ERGIC3 knockout HEK-293T cell line (Human ERGIC3 knockout HEK-293T cell line ab266489)

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: MCF-7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa

    Observed band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.

  • Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179), expandable thumbnail

    Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    All lanes: Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: K562 cell lysate at 10 µg

    Lane 5: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 43 kDa

    Observed band size: 50 kDa

  • OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179), expandable thumbnail

    OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (ab129179)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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