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AB129179

Anti-ERGIC3 antibody [EPR8141]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal ERGIC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

C20orf47, ERV46, SDBCAG84, CGI-54, PRO0989, ERGIC3, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (AB129179)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC3 antibody [EPR8141] (AB129179)

ab129179 at 1/100 staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (AB129179)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERGIC3 antibody [EPR8141] (AB129179)

ab129179 at 1/250 staining ERGIC3 in HeLa cells by Immunofluorescence.

Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179)
  • WB

Lab

Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179)

Lanes 1-4 : Merged signal (red and green). Green - ab129179 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266489 (knockout cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ERGIC3 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ERGIC3 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ergic3-knockout-hek-293t-cell-line-ab266489'>ab266489</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

MCF-7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179)
  • WB

Unknown

Western blot - Anti-ERGIC3 antibody [EPR8141] (AB129179)

All lanes:

Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 50 kDa

false

OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (AB129179)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ERGIC3 antibody [EPR8141] (AB129179)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-ERGIC3 antibody [EPR8141] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8141

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ERGIC3 protein also known as ERGIC-53 plays a critical role in the early secretory pathway. It is involved in vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus. Weighing around 38 kDa ERGIC3 is an important contributor to cargo transport processes. The protein finds expression in various tissues predominantly within cells that have high secretory demands.
Biological function summary

The ERGIC3 protein functions as part of the larger ER-Golgi intermediate compartment (ERGIC) complex helping in the sorting and transport of proteins. The ERGIC serves as an essential hub in the secretory pathway modulating cargo movement to the Golgi for further processing. Some studies have implicated ERGIC3 in maintaining Golgi integrity and optimal function affecting overall cellular health.

Pathways

ERGIC3 plays a significant role in the secretory pathway assisting in the transit of proteins and the correct folding of polypeptides. It links functionally to other key proteins such as KI67 which associates with cell proliferation regions and relies heavily on efficient protein processing for cellular division. The association suggests ERGIC3’s involvement in maintaining cellular growth and regeneration.

Researchers have connected ERGIC3 with diseases like congenital disorders of glycosylation due to its role in protein folding and trafficking. Furthermore disruptions in ERGIC3 function have correlations with cancer progression particularly in overactive secretion conditions. These disorders often coincide with aberrant expression or mutations in folding-related proteins signifying potential ERGIC3 involvement in pathological manifestations when its function is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed : 31142615).
See full target information ERGIC3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of genomics 2025:1575734 PubMed40881277

2025

Exploration of the Roles and Mechanisms Between Tumor-Infiltrating Lymphocytes and Hepatocellular Carcinoma Based on Single-Cell Transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Taiyu Shi,Donghao Cheng,Xinyan Du,Jianning Wang,Siyuan Jiang,Liangyu Yang,Fen Chen,Chenxu Gong,Yan Ou,Bangjie Chen,Yiwen Jia

Aging cell 24:e70105 PubMed40371663

2025

hTERT Increases TRF2 to Induce Telomere Compaction and Extend Cell Replicative Lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy Adam,Yang Yang,Mahbod Djamshidi,Sara Seifan,Nicholas S Y Ting,Joel Glover,Nicolas Touret,Paul M K Gordon,K V Vineetha Warriyar,Hokan Krowicki,Christine Kim Garcia,Sharon A Savage,Aaron A Goodarzi,Duncan M Baird,Tara L Beattie,Karl Riabowol

Cellular and molecular life sciences : CMLS 81:431 PubMed39395036

2024

Regulation of Cx36 trafficking through the early secretory pathway by COPII cargo receptors and Grasp55.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan Tetenborg,Fatemeh Ariakia,Elizabeth Martinez-Soler,Eyad Shihabeddin,Ignacio Cebrian Lazart,Adam C Miller,John O'Brien

Nature biotechnology 40:1132-1142 PubMed35468954

2022

A highly photostable and bright green fluorescent protein.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiko Hirano,Ryoko Ando,Satoshi Shimozono,Mayu Sugiyama,Noriyo Takeda,Hiroshi Kurokawa,Ryusaku Deguchi,Kazuki Endo,Kei Haga,Reiko Takai-Todaka,Shunsuke Inaura,Yuta Matsumura,Hiroshi Hama,Yasushi Okada,Takahiro Fujiwara,Takuya Morimoto,Kazuhiko Katayama,Atsushi Miyawaki

Traffic (Copenhagen, Denmark) 23:140-157 PubMed34994051

2022

ERGIC2 and ERGIC3 regulate the ER-to-Golgi transport of gap junction proteins in metazoans.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Guan,Yongzhi Yang,Jingjing Liang,Yue Miao,Angyang Shang,Baolei Wang,Yingchun Wang,Mei Ding

Journal of cell science 133: PubMed32079661

2020

TLR7 trafficking and signaling in B cells is regulated by the MHCII-associated invariant chain.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Tohme,Lucie Maisonneuve,Karim Achour,Michaël Dussiot,Sophia Maschalidi,Bénédicte Manoury

The Journal of biological chemistry 294:10900-10912 PubMed31142615

2019

The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Wonjin Yoo,Eun-Bee Cho,Sungjoo Kim,Jong-Bok Yoon
View all publications
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Product promise

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