Rabbit Recombinant Monoclonal ERGIC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615).
C20orf47, ERV46, SDBCAG84, CGI-54, PRO0989, ERGIC3, Endoplasmic reticulum-Golgi intermediate compartment protein 3, Serologically defined breast cancer antigen NY-BR-84
Rabbit Recombinant Monoclonal ERGIC3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The ERGIC3 protein also known as ERGIC-53 plays a critical role in the early secretory pathway. It is involved in vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus. Weighing around 38 kDa ERGIC3 is an important contributor to cargo transport processes. The protein finds expression in various tissues predominantly within cells that have high secretory demands.
The ERGIC3 protein functions as part of the larger ER-Golgi intermediate compartment (ERGIC) complex helping in the sorting and transport of proteins. The ERGIC serves as an essential hub in the secretory pathway modulating cargo movement to the Golgi for further processing. Some studies have implicated ERGIC3 in maintaining Golgi integrity and optimal function affecting overall cellular health.
ERGIC3 plays a significant role in the secretory pathway assisting in the transit of proteins and the correct folding of polypeptides. It links functionally to other key proteins such as KI67 which associates with cell proliferation regions and relies heavily on efficient protein processing for cellular division. The association suggests ERGIC3’s involvement in maintaining cellular growth and regeneration.
Researchers have connected ERGIC3 with diseases like congenital disorders of glycosylation due to its role in protein folding and trafficking. Furthermore disruptions in ERGIC3 function have correlations with cancer progression particularly in overactive secretion conditions. These disorders often coincide with aberrant expression or mutations in folding-related proteins signifying potential ERGIC3 involvement in pathological manifestations when its function is compromised.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
ab129179 Anti-ERGIC3 antibody [EPR8141] was shown to specifically react with ERGIC3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ERGIC3 knockout HEK-293T cell line ab266489 (knockout cell lysate Human ERGIC3 knockout HEK-293T cell lysate ab257941) was used. Wild-type and ERGIC3 knockout samples were subjected to SDS-PAGE. ab129179 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: ERGIC3 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human ERGIC3 knockout HEK-293T cell line (Human ERGIC3 knockout HEK-293T cell line ab266489)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: MCF-7 cell lysate at 20 µg
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa
ab129179, at 1/100, staining ERGIC3 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab129179, at 1/250, staining ERGIC3 in HeLa cells by Immunofluorescence.
All lanes: Western blot - Anti-ERGIC3 antibody [EPR8141] (ab129179) at 1/10000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
Lane 5: HeLa cell lysate at 10 µg
All lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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