AB166620

Anti-ERH antibody [EPR10830(B)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal ERH antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Enhancer of rudimentary homolog, ERH

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (AB166620)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling ERH with ab166620 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] (AB166620)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunofluorescence analysis of A431 cells labeling ERH with ab166620 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ERH with ab166620 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunoprecipitation analysis of ERH in pellet from A549 cell lysate using ab166620 at 1/10 dilution, followed by WB detection.

All lanes:

Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from A549 cell lysate at 10 µg

Predicted band size: 12 kDa

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Unknown

Western blot - Anti-ERH antibody [EPR10830(B)] (AB166620)

All lanes:

Western blot - Anti-ERH antibody [EPR10830(B)] (ab166620) at 1/1000 dilution

Lane 1:

T-47D cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 12 kDa

false

Carrier free

Anti-ERH antibody [EPR10830(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10830(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERH short for Enhancer of Rudimentary Homolog is a small protein involved in transcriptional processes. Also known in scientific literature by its alias 'e 'r 'h ' it has a molecular weight of approximately 12 kDa. ERH is widely expressed in various tissues indicating its broad role in cellular function. The protein contains two conserved domains that contribute to its ability to interact with other proteins in cellular processes.

Biological function summary

ERH participates in transcription and RNA processing functions through its involvement with RNA binding. ERH forms part of a multi-protein complex that includes other components such as mRNA splicing factors. Its role is essential in ensuring the proper processing of precursor mRNA molecules into mature mRNAs. Such molecular functions highlight ERH's importance in maintaining the efficiency of transcription and post-transcriptional modifications.

Pathways

Researchers have observed ERH's participation in gene expression and cell cycle pathways. Within these pathways ERH interacts with proteins like DIDO1 and SMARCA4 which are linked to chromatin remodeling and mRNA splicing respectively. The coordination of these proteins with ERH amplifies its significance in regulating gene expression and ensuring proper cell cycle progression.

ERH has connections to cancer and neurodevelopmental disorders. Alterations in ERH expression levels are associated with malignancies where its interaction with proteins such as LSF1 and SETDB1 can contribute to tumorigenesis. Similarly the disruption of ERH function links to neurodevelopmental disorders potentially through aberrant mRNA processing and gene expression regulation. These associations underline ERH as an important target for studying therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May have a role in the cell cycle.

See full target information ERH

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 44:3386-3406 PubMed40696167

2025

Glutaminase as a metabolic target of choice to counter acquired resistance to Palbociclib by colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Míriam Tarrado-Castellarnau,Carles Foguet,Josep Tarragó-Celada,Marc Palobart,Claudia Hernández-Carro,Jordi Perarnau,Erika Zodda,Ibrahim H Polat,Silvia Marin,Alejandro Suarez-Bonnet,Juan José Lozano,Mariia Yuneva,Timothy M Thomson,Marta Cascante

Nucleic acids research 53: PubMed40586312

2025

ERH regulates type II interferon immune signaling through post-transcriptional regulation of JAK2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Soderholm,Milica Vunjak,Melanie de Almeida,Niko Popitsch,Nadezda Podvalnaya,Pablo Araguas-Rodriguez,Sara Scinicariello,Emily Nischwitz,Falk Butter,René F Ketting,Stefan L Ameres,Michaela Müller-McNicoll,Johannes Zuber,Gijs A Versteeg

Nucleic acids research 51:3130-3149 PubMed36772830

2023

Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hala Al Adhami,Judith Vallet,Celia Schaal,Paul Schumacher,Anaïs Flore Bardet,Michael Dumas,Johana Chicher,Philippe Hammann,Sylvain Daujat,Michael Weber

Oncogene 41:125-137 PubMed34711954

2021

Menin inhibition suppresses castration-resistant prostate cancer and enhances chemosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Chaïma Cherif,Dang Tan Nguyen,Clément Paris,Thi Khanh Le,Thibaud Sefiane,Nadine Carbuccia,Pascal Finetti,Max Chaffanet,Abdessamad El Kaoutari,Julien Vernerey,Ladan Fazli,Martin Gleave,Mohamed Manai,Philippe Barthélémy,Daniel Birnbaum,François Bertucci,David Taïeb,Palma Rocchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERH antibody [EPR10830(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] (AB166620)

Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (AB166620)

Western blot - Anti-ERH antibody [EPR10830(B)] (AB166620)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise