Rabbit Recombinant Monoclonal ERH antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112512.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359)")
![Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--western-blot-img32727.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116032.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359)")
![Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunoprecipitation-img59757.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359)")
![Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunocytochemistry-immunofluorescence-img20475.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (AB249359)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Predicted | Not recommended | Predicted |
| Rat | Predicted | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Alternative names
Enhancer of rudimentary homolog, ERH
Recommended products
Rabbit Recombinant Monoclonal ERH antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10830(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249359 is the carrier-free version of Anti-ERH antibody [EPR10830(B)] ab166620.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERH short for Enhancer of Rudimentary Homolog is a small protein involved in transcriptional processes. Also known in scientific literature by its alias 'e 'r 'h ' it has a molecular weight of approximately 12 kDa. ERH is widely expressed in various tissues indicating its broad role in cellular function. The protein contains two conserved domains that contribute to its ability to interact with other proteins in cellular processes.
- Biological function summary
ERH participates in transcription and RNA processing functions through its involvement with RNA binding. ERH forms part of a multi-protein complex that includes other components such as mRNA splicing factors. Its role is essential in ensuring the proper processing of precursor mRNA molecules into mature mRNAs. Such molecular functions highlight ERH's importance in maintaining the efficiency of transcription and post-transcriptional modifications.
- Pathways
Researchers have observed ERH's participation in gene expression and cell cycle pathways. Within these pathways ERH interacts with proteins like DIDO1 and SMARCA4 which are linked to chromatin remodeling and mRNA splicing respectively. The coordination of these proteins with ERH amplifies its significance in regulating gene expression and ensuring proper cell cycle progression.
- Associated diseases and disorders
ERH has connections to cancer and neurodevelopmental disorders. Alterations in ERH expression levels are associated with malignancies where its interaction with proteins such as LSF1 and SETDB1 can contribute to tumorigenesis. Similarly the disruption of ERH function links to neurodevelopmental disorders potentially through aberrant mRNA processing and gene expression regulation. These associations underline ERH as an important target for studying therapeutic interventions in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112512.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
This data was developed using Anti-ERH antibody [EPR10830(B)] ab166620, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling ERH with Anti-ERH antibody [EPR10830(B)] ab166620 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--western-blot-img32727.jpg "Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Western blot - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
This data was developed using Anti-ERH antibody [EPR10830(B)] ab166620, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ERH antibody [EPR10830(B)] (Anti-ERH antibody [EPR10830(B)] ab166620) at 1/1000 dilution
Lane 1: T-47D cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
Predicted band size: 12 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116032.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
This data was developed using Anti-ERH antibody [EPR10830(B)] ab166620, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ERH with Anti-ERH antibody [EPR10830(B)] ab166620 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunoprecipitation-img59757.jpg "Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
This data was developed using Anti-ERH antibody [EPR10830(B)] ab166620, the same antibody clone in a different buffer formulation.
Immunoprecipitation analysis of ERH in pellet from A549 cell lysate using Anti-ERH antibody [EPR10830(B)] ab166620 at 1/10 dilution, followed by WB detection.
All lanes: Immunoprecipitation - Anti-ERH antibody [EPR10830(B)] (Anti-ERH antibody [EPR10830(B)] ab166620) at 1/1000 dilution
All lanes: immunoprecipitation pellet from A549 cell lysate at 10 µg
Predicted band size: 12 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359), expandable thumbnail](https://content.abcam.com/products/images/erh-antibody-epr10830b-bsa-and-azide-free-ab249359--immunocytochemistry-immunofluorescence-img20475.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)")
Immunocytochemistry/ Immunofluorescence - Anti-ERH antibody [EPR10830(B)] - BSA and Azide free (ab249359)
This data was developed using Anti-ERH antibody [EPR10830(B)] ab166620, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A431 cells labeling ERH with Anti-ERH antibody [EPR10830(B)] ab166620 at 1/250 dilution.
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