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Rabbit Recombinant Monoclonal ERK1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ERK1 antibody [EP4967] - BSA and Azide free (AB214169), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody [EP4967] - BSA and Azide free (AB214169), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 antibody [EP4967] - BSA and Azide free (AB214169), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ERK1 antibody [EP4967] - BSA and Azide free (AB214169), expandable thumbnail
  • Western blot - Anti-ERK1 antibody [EP4967] - BSA and Azide free (AB214169), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376-Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

2 products for Alternative Version

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed:35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Alternative names

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

Recommended products

Rabbit Recombinant Monoclonal ERK1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP4967
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab214169 is the carrier-free version of Anti-ERK1 antibody [EP4967] ab109282.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ERK1 also known as MAPK3 is an extracellular signal-regulated kinase involved in transmitting signals from the cell surface to the nucleus. This protein has a molecular mass of about 44 kDa. ERK1 expresses in various tissue types with higher expression in the brain heart and skeletal muscle. Researchers often study ERK1 in the context of its role in cellular signaling due to its involvement in critical regulatory functions.

Biological function summary

ERK1 plays a significant role in cell cycle regulation differentiation and proliferation. It forms part of the MAPK signaling cascade becoming activated through a phosphorylation event. In its activated form ERK1 translocates to the nucleus where it phosphorylates target substrates. ERK1 often functions in conjunction with its homolog ERK2 to mediate these cellular processes marking it as an essential player in growth factor signaling.

Pathways

ERK1 functions primarily within the MAPK/ERK signaling pathway a major conduit for transmitting proliferative signals from growth factor receptors. ERK1 interacts with proteins like MEK1/2 which phosphorylate and activate ERK1 in response to extracellular stimuli. Another critical pathway involving ERK1 is the Ras-Raf-MEK-ERK cascade which regulates various cellular outcomes. This connection to the Ras family highlights its importance in signal transduction and reinforces its position in critical cellular processes.

Associated diseases and disorders

Aberrant activation of ERK1 connects to diseases such as cancer and cardiovascular disorders. In cancer the dysregulation of the MAPK/ERK pathway often through mutations affecting Ras or Raf proteins leads to uncontrolled cell proliferation. ERK1's involvement in cardiovascular diseases links to its role in hypertrophic signaling in cardiac cells where altered ERK1 activity can contribute to pathological cardiac remodeling. Understanding these interactions can aid in developing therapeutic strategies targeting the MAPK/ERK signaling pathway.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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