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Rabbit Recombinant Monoclonal ERK1 phospho T202 antibody. Suitable for WB and reacts with Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human MAPK1 phospho T185.

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Images

Western blot - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) Antibody [S05-2H9] (AB317377), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human MAPK1 phospho T185. Database link P28482

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Predicted
Rat
Tested
Chinese hamster
Tested

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Chinese hamster

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Targets

Mapk1 phospho T185, Erk1 phospho T202, Erk2 phospho T185

Alternative names

Rabbit Recombinant Monoclonal ERK1 phospho T202 antibody. Suitable for WB and reacts with Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human MAPK1 phospho T185.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human MAPK1 phospho T185. Database link P28482
Clone number

S05-2H9

Purification technique

Affinity purification

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) Antibody [S05-2H9] (ab317377), expandable thumbnail

    Western blot - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) Antibody [S05-2H9] (ab317377)

    All lanes: Western blot - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) Antibody [S05-2H9] (ab317377) at 1/1000 dilution

    Lane 1: C6 cell lysate

    Lane 2: CHO-K1 cell lysate

    Predicted band size: 42 kDa, 44 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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