Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human MAPK1 phospho T185 + Y187.
Images
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img182037.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)")
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img174927.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)")
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img175546.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)")
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img171722.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)")
![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunocytochemistry-immunofluorescence-img439874.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MAPK1 phospho T185 + Y187. The exact immunogen used to generate this antibody is proprietary information. Database link P28482
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed:35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Additional Targets
MAPK1 phospho T185 + Y187
Alternative names
ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK
Recommended products
Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human MAPK1 phospho T185 + Y187.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- Synthetic Peptide within Human MAPK1 phospho T185 + Y187. The exact immunogen used to generate this antibody is proprietary information. Database link P28482
- Clone number
- HL173
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.
- Biological function summary
ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.
- Pathways
ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.
- Associated diseases and disorders
Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img182037.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Whole cell extracts (30 μg) were separated by 10% SDS-PAGE
All lanes: Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367) at 1/1000 dilution
Lane 1: Starvation,16h untreated A431 whole cell extract at 30 µg
Lane 2: Starvation, 16h + 200ng/ml EGF, 30 min, treated A431 whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img174927.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Whole cell extracts (30 μg) were separated by 10% SDS-PAGE
All lanes: Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367) at 1000 µg
Lane 1: Untreated Neuro2A cells at 30 µg
Lane 2: 50um Ethacrynic acid, 2h treated Neuro2A cells at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img175546.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Whole cell extracts were separated by 10% SDS-PAGE
All lanes: Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367) at 1/500 dilution
Lane 1: Untreated PC-12 whole cell extract
Lane 2: 200 nm TPA, 30min treated PC-12 whole cell extract
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--western-blot-img171722.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Whole cell extracts were separated by 10% SDS-PAGE
All lanes: Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367) at 1/1000 dilution
Lane 1: Untreated Rat2 whole cell lysates at 30 µg
Lane 2: 50 μm Ethacrynic acid treated Rat2 whole cell lysates at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunocytochemistry-immunofluorescence-img439874.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Immunofluorescence analysis of 4% paraformaldehyde fixed NIH-3T3 cells labelling ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm using ab289367 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunocytochemistry-immunofluorescence-img439875.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated A431 cells labelling ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein using ab289367 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439876.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)
Paraffin-embedded human breast carcinoma tissue stained for ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at nucleus with ab289367 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunocytochemistry-immunofluorescence-img439875.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367), expandable thumbnail](https://content.abcam.com/products/images/erk1-phospho-t202-y204-erk2-phospho-t185-y187-antibody-hl173-bsa-and-azide-free-ab289367--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img439876.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (ab289367)")
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