AB223500

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327]

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(71 Publications)

Rabbit Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB and reacts with Human samples. Cited in 71 publications.

View Alternative Names

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK, ERK2, PRKM1, PRKM2, MAPK1, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

All lanes:

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500) at 1/400 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) + EGF cell lysate

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate

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CiteAb

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) western blot using anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] ab223500. Publication image and figure legend from Feng, P. C., Ke, X. F., et al., 2019, Stem Cell Res Ther, PubMed 31387619.

ab223500 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab223500 please see the product overview.

BMP2 regulates the activation of the MARK/p38 signaling pathway in endothelial cells of HCC. a Bands of total p38, ERK1/2, JNK, and Akt proteins and phosphorylated proteins in ECV304 cells measured by Western blot analysis after over-expression or silencing of BMP2. b Expression of p38, ERK1/2, JNK, and Akt proteins and their phosphorylation extents in ECV304 cells. Relative protein expression values were expressed as mean ± standard deviation, and data between two groups were compared by Student's t test. *p < 0.05. HCC, hepatocellular carcinoma; BMP2, bone morphogenetic proteins 2; NC, negative control; MAPK, mitogen-activated protein kinase

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Carrier free

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP327

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.

Biological function summary

ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.

Pathways

ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.

Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Phosphorylates GJA1 at 'Ser-279' and 'Ser-282' resulting in an increase in GJA1 ubiquitination and ultimately lysosomal degradation (By similarity).

See full target information MAPK3 pT202 + Y204

Additional targets

MAPK1 phospho T185 + Y187

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Publications (71)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 24:424 PubMed39709453

2024

Epidermal growth factor receptor ligands enriched in follicular fluid exosomes promote oncogenesis of fallopian tube epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aye Aye Khine,Pao-Chu Chen,Ying-Hsi Chen,Sung-Chao Chu,Hsuan-Shun Huang,Tang-Yuan Chu

Environmental toxicology 40:88-100 PubMed39268877

2024

The Novel Application of EUK-134 in Retinal Degeneration: Preventing Mitochondrial Oxidative Stress-Triggered Retinal Pigment Epithelial Cell Apoptosis by Suppressing MAPK/p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shang-Chun Tsou,Chen-Ju Chuang,Chin-Lin Hsu,Tzu-Chun Chen,Jui-Hsuan Yeh,Meilin Wang,Inga Wang,Yuan-Yen Chang,Hui-Wen Lin

Cell death discovery 10:308 PubMed38961068

2024

Plac8-ERK pathway modulation of monocyte function in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Zhang,Jing-Nan Fu,Gui-Bing Chen,Xiu Zhang

Journal of biomedical materials research. Part A 112:65-81 PubMed37723658

2023

Heparin-collagen I bilayers stimulate FAK/ERK½ signaling via α2β1 integrin to support the growth and anti-inflammatory potency of mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Said J Cifuentes,Maribella Domenech

Molecular carcinogenesis 62:1417-1427 PubMed37265438

2023

Insulin-like growth factor (IGF) and hepatocyte growth factor (HGF) in follicular fluid cooperatively promote the oncogenesis of high-grade serous carcinoma from fallopian tube epithelial cells: Dissection of the molecular effects.

Applications

Unspecified application

Species

Unspecified reactive species

Tang-Yuan Chu,Aye Aye Khine,Na-Yi Yuan Wu,Pao-Chu Chen,Sung-Chao Chu,Ming-Hsun Lee,Hsuan-Shun Huang

BMC cancer 23:364 PubMed37081411

2023

AC010883.5 promotes cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition in cervical cancer by modulating the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyu Gan,Xia Huang,Wenrong Zhao,Hui Liu,Yan Xu,Xiaohua Zhang,Jingxin Cheng,Rui Chen

Cellular oncology (Dordrecht) 46:987-1000 PubMed36917356

2023

STIM1-regulated exosomal EBV-LMP1 empowers endothelial cells with an aggressive phenotype by activating the Akt/ERK pathway in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yayan Deng,Xue Liu,Yujuan Huang,Jiaxiang Ye,Qian He,Yue Luo,Yong Chen,Qiuyun Li,Yan Lin,Rong Liang,Yongqiang Li,Jiazhang Wei,Jinyan Zhang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22782 PubMed36786721

2023

Exosomes derived from human adipose-derived stem cells alleviate hepatic ischemia-reperfusion (I/R) injury through the miR-183/ALOX5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Gong,Haisu Dai,Wei Liu,Rui Liao,Hailei Chen,Leida Zhang,Xiaojun Wang,Zhiyu Chen

Naunyn-Schmiedeberg's archives of pharmacology 396:1461-1470 PubMed36749400

2023

Dapagliflozin protects against dilated cardiomyopathy progression by targeting NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Hu,Jiamin Xu,Xi Tan,Dong Li,Dejiang Yao,Biao Xu,Yuhua Lei

Cell death and differentiation 30:766-778 PubMed36329235

2022

Blockage of PPARγ T166 phosphorylation enhances the inducibility of beige adipocytes and improves metabolic dysfunctions.

Applications

Unspecified application

Species

Unspecified reactive species

Nanfei Yang,Yuxin Wang,Qiang Tian,Qiuping Wang,Yan Lu,Luchen Sun,Sijie Wang,Yuncheng Bei,Jianguo Ji,Hu Zhou,Wei Yang,Pengju Yao,Wenyuan Zhu,Lingyun Sun,Zhifeng Huang,Xiaokun Li,Pingping Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (AB223500)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (71)

Product promise