Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/50 dilution, compared to a negative control without primary antibody (left).

To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1 : 50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus withab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).

To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1 : 20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).

To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• WB

Supplier Data

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Bands of 42 kDa and 44 kDa corresponding to Phospho-p44 MAPK + p42 MAPK pThr185 + pTyr187 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

All lanes:

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate, with treatment of EGF(100 ng/mL for 15 mins) at 30 µg

Lane 2:

MDA-MB-231 whole cell lysate at 30 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 42 kDa,44 kDa

false

• WB

Supplier Data

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Extracts of PC12 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.

The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with ab4819 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with :

Lane 1 and 2 : no peptide

Lane 3 : the non-phosphopeptide corresponding to the phosphopeptide immunogen

Lane 4 : a generic phosphothreonine-containing peptide

Lane 5 : a generic phosphotyrosine-containing peptide

Labe 6 : the phosphopeptide immunogen

Detection : Pierce SuperSignal™ method.

All lanes:

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate, unstimulated

Lanes 2 - 6:

PC-12 whole cell lysate, stimulated with 0.5 M sorbitol for 5 minutes

Secondary

All lanes:

Goat F (ab')2 anti-rabbit IgG HRP conjugate

Predicted band size: 42 kDa,44 kDa

false

• WB

Supplier Data

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (AB4819)

Data was analyzed on the LI-COR Odyssey® Infrared Imaging System.

All lanes:

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 whole cell lysate, treated with either PDGF at 20 µg

Secondary

All lanes:

Anti-rabbit secondary antibody conjugated to Alexa fluor 680

Predicted band size: 42 kDa,44 kDa

false