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Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for IP, Dot, WB, ELISA and reacts with Rat, Human, Mouse, Synthetic peptide samples. Cited in 87 publications.

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Images

Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299), expandable thumbnail
  • Dot Blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299), expandable thumbnail
  • ELISA - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299), expandable thumbnail
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299), expandable thumbnail
  • Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PFlow CytIPDotWBELISA
Human
Not recommended
Not recommended
Not recommended
Tested
Expected
Tested
Expected
Mouse
Not recommended
Not recommended
Not recommended
Expected
Expected
Tested
Expected
Rat
Not recommended
Not recommended
Not recommended
Tested
Expected
Tested
Expected
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Rat, Mouse, Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/80

Notes

-

Species

Human

Dilution info

1/80

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/5000 - 1/10000

Notes

-

Species

Human

Dilution info

1/5000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Targets

MAPK1 phospho Y187 + T185

Alternative names

Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK, PRKM3, ERK1, MAPK3

Recommended products

Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody. Suitable for IP, Dot, WB, ELISA and reacts with Rat, Human, Mouse, Synthetic peptide samples. Cited in 87 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP197Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects Erk1 phosphorylated at Threonine 202 and Tyrosine 204 and Erk2 phosphorylated at Threonine 185 and Tyrosine 187.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Erk1 and Erk2 also known as MAPK3 and MAPK1 are part of the mitogen-activated protein kinase family. These proteins play an important role in signal transduction. Erk1 has a mass of about 44 kDa while Erk2 is slightly smaller at approximately 42 kDa. Both proteins are widely expressed in a variety of tissues contributing to the transmission of signals from the cell surface to the DNA in the cell nucleus. Specifically Erk1 and Erk2 are activated through dual phosphorylation at threonine 202 and tyrosine 204 for Erk1 and threonine 185 and tyrosine 187 for Erk2.

Biological function summary

Erk1 and Erk2 are involved in several essential cellular processes including proliferation differentiation and survival. They often operate as part of a larger complex engaging with other proteins to drive cellular responses. Upon activation they move from the cytoplasm to the nucleus where they regulate the activity of various transcription factors. Erk signaling can adjust gene expression by phosphorylating nuclear targets which influences cell cycle progression and apoptosis.

Pathways

Both Erk1 and Erk2 mainly operate within the MAPK/ERK pathway. This signaling pathway is important for transmitting signals from growth factors and other extracellular stimulants. Related proteins such as Ras and Raf are upstream activators in the pathway. Downstream Erk proteins can impact other cascades including those governing cellular growth and division. By interacting with multiple proteins within these pathways Erk1 and Erk2 ensure accurate cellular responses to environmental changes.

Associated diseases and disorders

Alterations in the function of Erk1 and Erk2 can contribute to cancer development such as colorectal cancer and melanoma. Misregulation of Erk signaling can lead to uncontrolled cell proliferation and tumor growth. These proteins also connect to neurodegenerative disorders like Alzheimer's disease where disrupted Erk signaling may contribute to neuronal damage. Erk1 and Erk2 interact with other disease-related proteins like BRAF in cancer and APP in Alzheimer's highlighting their role in disease pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    ab76299 at 1/80 immunoprecipitating Erk1 (pT202/pY204) + Erk2 (pT185/pY187) in PC-12 whole cell lysate.

    Lane 1 (input): PC-12 whole cell lysate - treated with 100ng/ml NGF for 10 min (10μg).

    Lane 2 (+): ab76299 + PC-12 whole cell lysate - treated with 100ng/ml NGF for 10 min.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76299 in PC-12 whole cell lysate - treated with 100ng/ml NGF for 10 min.

    For western blotting, ab76299 was used at a dilution of 1/1000 and a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 1s

  • Dot Blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Dot Blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Dot blot analysis of single phospho peptide pT202 (Lane 1), single phospho peptide pY204 (Lane 2), double phospho peptide pT202/pY204 (Lane 3) and non-phospho peptide (Lane 4) labelling Erk1 (pT202/pY204) + Erk2 (p185/pY187) with ab76299 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/1000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • ELISA - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    ELISA - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    ELISA analysis of various phospho and non-phospho peptides (0-250 ng/ml) labelling Erk1 (pT202/pY204) + Erk2 (pT185/pY187) with ab76299 at a dilution of 1/1000. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    ab76299 has stronger affinity for the double phospho peptide Y204/Y187 than to single phospho peptides T202 or Y204.

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299) at 1/5000 dilution

    Lane 1: A431 whole cell lysate - untreated at 10 µg

    Lane 2: A431 whole cell lysate - treated with 100ng/ml EGF for 10 minutes at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 43 kDa

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    ab76299 at 1/80 immunoprecipitating Erk1 (pT202/pY204) + Erk2 (pT185/pY187) in A431 whole cell lysate.

    Lane 1 (input): A431 whole cell lysate - treated with 100ng/ml EGF for 10 min (10μg).

    Lane 2 (+): ab76299 + A431 whole cell lysate - treated with 100ng/ml EGF for 10 min.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76299 in A431 whole cell lysate - treated with 100ng/ml EGF for 10 min.

    For western blotting, ab76299 was used at a dilution of 1/1000 and a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG, was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 3s

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299) at 1/10000 dilution

    Lane 1: SH-SY5Y whole cell lysate - untreated at 10 µg

    Lane 2: SH-SY5Y whole cell lysate - treated with 100ng/ml NGF for 10 minutes at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 43 kDa

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 15s

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299) at 1/10000 dilution

    Lane 1: PC-12 whole cell lysate - untreated at 10 µg

    Lane 2: PC-12 whole cell lysate - treated with 100ng/ml NGF for 10 minutes at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 43 kDa

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 15s

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299) at 1/10000 dilution

    Lane 1: HeLa whole cell lysate - untreated at 10 µg

    Lane 2: HeLa whole cell lysate - treated with Lambda Phosphatase at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 43 kDa

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 30s

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299), expandable thumbnail

    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (ab76299) at 1/10000 dilution

    All lanes: NIH/3T3 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 41 kDa, 43 kDa

    Observed band size: 42 kDa, 44 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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