JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227134

Anti-ERK2 antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal ERK2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Dog, Cat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK1.

View Alternative Names

ERK2, PRKM1, PRKM2, MAPK1, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2

7 Images
Immunoprecipitation - Anti-ERK2 antibody (AB227134)
  • IP

Supplier Data

Immunoprecipitation - Anti-ERK2 antibody (AB227134)

ERK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 μg ab227134. Western blot was performed from the immunoprecipitate using ab227134 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent.

Lane 1 : HeLa whole cell extract 30 μg.

Lane 2 : Control IP in HeLa whole cell extract with 4 μg of preimmune Rabbit IgG.

Lane 3 : ab227134 IP in HeLa whole cell extract.

10% SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-ERK2 antibody (ab227134)

Predicted band size: 41 kDa

false

Western blot - Anti-ERK2 antibody (AB227134)
  • WB

Supplier Data

Western blot - Anti-ERK2 antibody (AB227134)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERK2 antibody (ab227134) at 1/1000 dilution

Lane 1:

Wild-type ERK2 HeLa cell extracts at 30 µg

Lane 2:

Knockout ERK2 HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 41 kDa

false

Western blot - Anti-ERK2 antibody (AB227134)
  • WB

Supplier Data

Western blot - Anti-ERK2 antibody (AB227134)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ERK2 antibody (ab227134) at 1/1000 dilution

Lane 1:

H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Western blot - Anti-ERK2 antibody (AB227134)
  • WB

Supplier Data

Western blot - Anti-ERK2 antibody (AB227134)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ERK2 antibody (ab227134) at 1/5000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 41 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody (AB227134)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody (AB227134)

Immunohistochemical analysis of paraffin-embedded cat mammary gland tissue stained for ERK2 protein at cytoplasm and nucleus with ab227134 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-ERK2 antibody (AB227134)
  • WB

Supplier Data

Western blot - Anti-ERK2 antibody (AB227134)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERK2 antibody (ab227134) at 1/1000 dilution

All lanes:

PG-4 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 41 kDa

false

Western blot - Anti-ERK2 antibody (AB227134)
  • WB

Supplier Data

Western blot - Anti-ERK2 antibody (AB227134)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERK2 antibody (ab227134) at 1/1000 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Dog, Human, Cat

Applications

IHC-P, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human MAPK1. The exact immunogen used to generate this antibody is proprietary information.

P28482

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.
Biological function summary

ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.

Pathways

ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.

ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
See full target information MAPK1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40214468

2025

A Broad-Spectrum Chemokine Inhibitor Drives M2 Macrophage Polarization Through Modulation of the Myometrial Secretome.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Boros-Rausch,Anna Dorogin,Lubna Nadeem,Oksana Shynlova,Stephen James Lye

Scientific reports 14:12090 PubMed38802444

2024

Micropeptide AF127577.4-ORF hidden in a lncRNA diminishes glioblastoma cell proliferation via the modulation of ERK2/METTL3 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Baoshun Du,Zheying Zhang,Linlin Jia,Huan Zhang,Shuai Zhang,Haijun Wang,Zhenguo Cheng

The journal of gene medicine 24:e3395 PubMed34668273

2022

EZH2 upregulates the expression of MAPK1 to promote intervertebral disc degeneration via suppression of miR-129-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhou,Shuang-Jun He,Wei Liu,Mao-Jie Yang,Zhen-Yang Hou,Qian Meng,Zhong-Lai Qian

The Journal of experimental medicine 219: PubMed34825915

2021

WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ensong Guo,Rourou Xiao,Yifan Wu,Funian Lu,Chen Liu,Bin Yang,Xi Li,Yu Fu,Zizhuo Wang,Yuan Li,Yuhan Huang,Fuxia Li,Xue Wu,Lixin You,Tianyu Qin,Yiling Lu,Xiaoyuan Huang,Ding Ma,Gordon B Mills,Chaoyang Sun,Gang Chen

Molecular medicine reports 17:7170-7176 PubMed29568944

2018

PEDF regulates lipid metabolism and reduces apoptosis in hypoxic H9c2 cells by inducing autophagy related 5-mediated autophagy via PEDF-R.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqian Zhang,Yufeng Li,Zhiwei Liu,Qixiang Zhao,Hao Zhang,Xiaoyu Wang,Peng Lu,Hongli Yu,Meng Wang,Hongyan Dong,Zhongming Zhang
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB269567

Anti-ERK2 antibody [1B3B9] - BSA and Azide free

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [1B3B9] - BSA and Azide free (AB269567)

  • 0
  • 0
Primary Antibodies

AB205819

HRP Anti-ERK2 antibody [E460]

RabMAb|Recombinant|KO Validated

Western blot - HRP Anti-ERK2 antibody [E460] (AB205819)

  • 0
  • 0
Primary Antibodies

AB15282

Anti-ERK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody (AB15282)

  • 0
  • 0
Primary Antibodies

AB32081

Anti-ERK2 antibody [E460]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] (AB32081)

  • 5
  • 2
Primary Antibodies

AB231085

Anti-ERK2 antibody [1B3B9]

BOND RX™ Validated|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [1B3B9] (AB231085)

  • 0
  • 0
Primary Antibodies

AB190581

Alexa Fluor® 647 Anti-ERK2 antibody [E460]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-ERK2 antibody [E460] (AB190581)

  • 0
  • 0
Primary Antibodies

AB214170

Anti-ERK2 antibody [E460] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170)

  • 0
  • 0

Complementary Products

Assay Kits

AB317550

Human/Mouse /Rat ERK2 ELISA Kit

SimpleStep

Sandwich ELISA - Human/Mouse /Rat ERK2 ELISA Kit (AB317550)

  • 0
  • 0
Primary Antibodies

AB201015

Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] (AB201015)

  • 4
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com