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Rabbit Recombinant Monoclonal ERK2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170), expandable thumbnail
  • Western blot - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] - BSA and Azide free (AB214170), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Expected
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Tested
Tested
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Can be blocked with ab205612.

Species
Rat
Dilution info
-
Notes

Can be blocked with ab205612.

Species
Human
Dilution info
-
Notes

Can be blocked with ab205612.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

2 products for Alternative Version

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity). Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Alternative names

ERK2, PRKM1, PRKM2, MAPK1, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2

Recommended products

Rabbit Recombinant Monoclonal ERK2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
E460
Purification technique
Affinity purification Protein A
Epitope
ab32081 reacts with an epitope located in the C terminal region of ERK2.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab214170 is the carrier-free version of Anti-ERK2 antibody [E460] ab32081.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ERK2 also known as Extracellular signal-Regulated Kinase 2 is a serine/threonine protein kinase in the mitogen-activated protein kinase (MAPK) family with a mass of approximately 42 kDa. This kinase is expressed in many cell types and tissues including the brain liver and lungs. ERK2 plays a significant role in cellular processes such as proliferation differentiation and survival. It is often analyzed using specific assays including ERK2 ELISA and examination of cell lysate samples to determine expression levels and activity.

Biological function summary

ERK2 influences several key cellular functions. It functions as part of a signaling cascade transmitting signals from the exterior to the cell nucleus. In this cascade ERK2 is often part of a multi-protein complex that undergoes sequential phosphorylation. Through these mechanisms ERK2 regulates gene expression and is a pivotal component of the MAPK/ERK pathway ensuring the proper response to growth signals and stress stimuli.

Pathways

ERK2 is a central component of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways play critical roles in cell cycle regulation and apoptosis. ERK2 activation leads to its interaction with the MEK1/2 proteins which further allows the transmission of mitogenic signals. The interplay between ERK2 and related proteins like E460 often impacts cellular growth and development as it precisely controls the phosphorylation events within the pathway.

Associated diseases and disorders

ERK2 is connected to certain types of cancer and neurodegenerative diseases. Aberrations in ERK2 signaling pathways are often linked to tumorigenesis where altered interaction with proteins such as Raf and MEK1/2 disrupts cell cycle regulation and apoptosis. In neurodegenerative disorders dysregulated ERK2 activity has been associated with proteins contributing to Alzheimer’s disease indicating its involvement in neuronal survival and stress response mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail
    Image from Emmanuel C et al. PLoS One. 2011 Mar 15;6(3):e17617. doi: 10.1371/journal.pone.0017617.; Fig 2.; March 15 2011 PLoS ONE 6(3): e17617.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with Anti-ERK2 antibody [E460] ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ERK2 antibody [E460] ab32081).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Western blot - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-ERK2 antibody [E460] ab32081).

    Lanes 1-2: Merged signal (red and green). Green - Anti-ERK2 antibody [E460] ab32081 observed at 41 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    Anti-ERK2 antibody [E460] ab32081 Anti-ERK2 antibody [E460] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MAPK1 (ERK2) knockout HeLa cell line ab265052 (knockout cell lysate Human MAPK1 (ERK2) knockout HeLa cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. Anti-ERK2 antibody [E460] ab32081 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: MAPK1 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (Human MAPK1 (ERK2) knockout HeLa cell line ab265052)

    Performed under reducing conditions.

    Predicted band size: 41 kDa

    Observed band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    IHC image of Anti-ERK2 antibody [E460] ab32081 staining ERK2 in rat pancreas formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Anti-ERK2 antibody [E460] ab32081, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ERK2 antibody [E460] ab32081).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ERK2 with Anti-ERK2 antibody [E460] ab32081 at 1/400. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ERK2 antibody [E460] ab32081).

  • Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    Overlay histogram showing HeLa cells stained with Anti-ERK2 antibody [E460] ab32081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-ERK2 antibody [E460] ab32081, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ERK2 antibody [E460] ab32081).

  • Immunocytochemistry/ Immunofluorescence - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    Anti-ERK2 antibody [E460] ab32081 staining ERK2 in wild-type HeLa cells (top panel) and ERK2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-ERK2 antibody [E460] ab32081 at 0.2µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170), expandable thumbnail

    Western blot - Anti-ERK2 antibody [E460] - BSA and Azide free (ab214170)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-ERK2 antibody [E460] ab32081)


    Anti-ERK2 antibody [E460] ab32081 was shown to react with MAPK1 in wild-type HeLa cells in Western blot with loss of signal observed in MAPK1 knockout cell line Human MAPK1 (ERK2) knockout HeLa cell line ab265052. Wild-type HeLa and MAPK1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-ERK2 antibody [E460] ab32081 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-ERK2 antibody [E460] (Anti-ERK2 antibody [E460] ab32081) at 1/1000 dilution

    Lane 1: Wild-type HeLa lysate at 20 µg

    Lane 2: Western blot - Human MAPK1 (ERK2) knockout HeLa cell line (Human MAPK1 (ERK2) knockout HeLa cell line ab265052) at 20 µg

    Observed band size: 41 kDa

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