AB232748

Anti-ERK5 antibody

Name: Anti-ERK5 antibody (ab232748) | Abcam
Brand: Abcam Limited
SKU: AB232748
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ERK5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK7 aa 550-800.

View Alternative Names

BMK1, ERK5, PRKM7, MAPK7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, Extracellular signal-regulated kinase 5, BMK-1, ERK-5

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human colorectal cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human lung cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human kidney tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 88 kDa

false

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Recombinant human ERK5 protein

Predicted band size: 26 kDa,88 kDa

false

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Pig colon tissue lysate

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAPK7 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

Q13164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232748 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.

Biological function summary

ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.

Pathways

ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.

Abnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

See full target information MAPK7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:402-411 PubMed39633051

2024

Fetal hepatocytes protect the HSPC genome via fetuin-A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lin Guo,Yi-Ding Wang,Yan-Jun Liu,Lei Chu,Hua Zhu,Ye Hu,Ren-Yan Wu,Hong-Yu Xie,Juan Yu,Shui-Ping Li,Chao-Yang Xiong,Ruo-Yan Li,Fang Ke,Lei Chen,Guo-Qiang Chen,Liang Chen,Fan Bai,Tariq Enver,Guo-Hong Li,Huai-Fang Li,Deng-Li Hong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)

Western blot - Anti-ERK5 antibody (AB232748)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise