Name: Anti-ERK5 antibody [EP791Y]
SKU: ab40809
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal ERK5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

Images

Immunoprecipitation - Anti-ERK5 antibody [EP791Y] (AB40809), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes For unpurified use at 1/50 dilution.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10000	Notes For unpurified use at 1/1000 - 1/5000 dilution.
Species Mouse	Dilution info 1/10000	Notes For unpurified use at 1/1000 - 1/5000 dilution.
Species Rat	Dilution info 1/10000	Notes For unpurified use at 1/1000 - 1/5000 dilution.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes For unpurified use at 1/250 - 1/500 dilution.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/100 dilution.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information MAPK7

Function

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.

Alternative names

BMK1, ERK5, PRKM7, MAPK7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, Extracellular signal-regulated kinase 5, BMK-1, ERK-5

Recommended products

Rabbit Recombinant Monoclonal ERK5 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP791Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.

Biological function summary

ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.

Pathways

ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.

Associated diseases and disorders

Abnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunoprecipitation - Anti-ERK5 antibody [EP791Y] (ab40809)
ab40809 (purified) at 1:30 dilution (2ug) immunoprecipitating ERK5 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab40809 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab40809 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-ERK5 antibody [EP791Y] (ab40809)
Predicted band size: 88 kDa
Observed band size: 115 kDa
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Lanes 1- 2: Merged signal (red and green). Green - ab40809 observed at 115 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
ab40809 was shown to react with ERK5 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human MAPK7 (ERK5) knockout HeLa cell line ab265508 (knockout cell lysate Human MAPK7 (ERK5) knockout HeLa cell lysate ab258042) was used. Wild-type HeLa and MAPK7 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab40809 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERK5 antibody [EP791Y] (ab40809) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MAPK7 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human MAPK7 (ERK5) knockout HeLa cell line (Human MAPK7 (ERK5) knockout HeLa cell line ab265508)
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 115 kDa
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
ERK5 Western blot staining using rabbit Anti-ERK5 antibody
All lanes: Western blot - Anti-ERK5 antibody [EP791Y] (ab40809) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 115 kDa
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: MAPK7 (ERK5) knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lanes 1 - 3: Merged signal (red and green). Green - ab40809 observed at 88 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Unpurified ab40809 was shown to specifically react with ERK5 in wild-type HAP1 cells as signal was lost in MAPK7 (ERK5) knockout cells. Wild-type and MAPK7 (ERK5) knockout samples were subjected to SDS-PAGE. Unpurified ab40809 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Predicted band size: 88 kDa
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
The predicted weight of 89 kDa is for the precursor version of human ERK5 protein. However, ab40809 detects endogenous levels of total Erk5 protein which appears around 115 kDa in SDS PAGE
All lanes: Western blot - Anti-ERK5 antibody [EP791Y] (ab40809) at 1/5000 dilution
All lanes: Hela cell lysate at 10 µg
Predicted band size: 88 kDa
Observed band size: 115 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ERK5 antibody [EP791Y] (ab40809)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ERK5 with Purified ab40809 at 1:100 (4.8 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-ERK5 antibody [EP791Y] (ab40809)
Overlay histogram showing A549 cells stained with unpurified ab40809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40809, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, Anti-ERK5 antibody [EP791Y] - Total protein control (ab40809) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ERK5 (phospho T733) antibody [EPR28258-101] (Anti-ERK5 (phospho T733) antibody [EPR28258-101] ab323508) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells co-transfected with a human ERK5 WT expression vector containing a myc-His-tag® and a rat MEK5(S311D/T315D mutation)) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg with NFDM/TBST
Lane 2: 293T (human embryonic kidney epithelial cell) cells co-transfected with a human ERK5 (T733A mutation) expression vector containing a myc-His-tag® and a rat MEK5(S311D/T315D mutation)) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 115 kDa
Exposure time: 70s
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-ERK5 (phospho T733) antibody [EPR28258-101] ab323508 was shown to bind specifically to ERK5 (phospho T733). Target of interest was observed at 115 kDa in treated wild-type HeLa cell lysates (lanes -2-4) with no signal observed at this size in treated ERK5 knockout cell line (lanes 6-8) (lanes 5-8, knockout cell line Human MAPK7 (ERK5) knockout HeLa cell line ab265508 / knockout cell lysate Human MAPK7 (ERK5) knockout HeLa cell lysate ab258042).
Lane 1-8 on the left: untreated membrane;

Lane 1-8 on the right: membrane treated with alkaline phosphatase.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-ERK5 antibody [EP791Y] - Total protein control (ab40809) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ERK5 (phospho T733) antibody [EPR28258-101] (Anti-ERK5 (phospho T733) antibody [EPR28258-101] ab323508) at 1/1000 dilution
Lane 1: Untreated parental HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: Parental HeLa treated with 0.4M Sorbitol for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 3: Parental HeLa treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 4: Parental HeLa treated with 0.4M Sorbitol and 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 5: Untreated ERK5 knockout HeLa whole cell lysate at 20 µg with NFDM/TBST
Lane 6: ERK5 knockout HeLa treated with 0.4M Sorbitol for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 7: ERK5 knockout HeLa treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 8: ERK5 knockout HeLa treated with 0.4M Sorbitol and 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 115 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-ERK5 antibody [EP791Y] (ab40809)
Lane 1-4 on the left: untreated membrane;

Lane 1-4 on the right: membrane treated with alkaline phosphatase.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-ERK5 antibody [EP791Y] - Total protein control (ab40809) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ERK5 (phospho T733) antibody [EPR28258-101] (Anti-ERK5 (phospho T733) antibody [EPR28258-101] ab323508) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: NIH/3T3 treated with 0.4M Sorbitol for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 3: NIH/3T3 treated with 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Lane 4: NIH/3T3 treated with 0.4M Sorbitol and 100nM Calyculin A for 30min whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 115 kDa
Exposure time: 180s
Flow Cytometry (Intracellular) - Anti-ERK5 antibody [EP791Y] (ab40809)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ERK5 with purified ab40809 at 1/50 dilution (10 ug/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluorr^®488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).