Rabbit Polyclonal ERK8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK15 aa 1-250.
Images
Publications
- The European journal of neuroscience 55:1471-14822022Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Huang,Pei Wang,Haijun Liu,Mingjian Li,Yujiao Yue,Ping XuPubMed 35243702
- Journal of cellular biochemistry 122:16-282020REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanan Luan,Bin Xie,Wenbin WeiPubMed 32965043
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAPK15 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TD08
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).
Alternative names
Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, Extracellular signal-regulated kinase 8, ERK-7, ERK-8, MAPK15, ERK7, ERK8
Recommended products
Rabbit Polyclonal ERK8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK15 aa 1-250.
Alternative names
Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, Extracellular signal-regulated kinase 8, ERK-7, ERK-8, MAPK15, ERK7, ERK8
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAPK15 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TD08
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
ERK8 also known as MAPK15 is a serine/threonine kinase with a molecular weight of approximately 49 kDa. It has an important role in cellular signaling pathways. This protein is involved in the regulation of several cellular processes including growth proliferation and survival. ERK8 is expressed in various tissues with notable expression in the brain lung and liver. Due to its broad expression profile it holds importance in understanding diverse physiological and pathological contexts.
- Biological function summary
ERK8 engages in the regulation of cellular processes by modulating the activity of downstream targets. It is part of the Mitogen-Activated Protein Kinase (MAPK) family which typically functions in concert with other proteins to form signaling complexes. These complexes carry out phosphorylation events that are important for signaling transduction. ERK8 affects cell motility cytoskeletal rearrangements and transcriptional regulation impacting processes critical for cellular homeostasis and response to stress.
- Pathways
ERK8 is involved in signaling pathways including the MAPK/ERK pathway and the PI3K/AKT pathway. Within these pathways ERK8 interacts closely with proteins such as RAF and MEK facilitating the transmission of extracellular signals to intracellular responses. Its role in the MAPK/ERK pathway is particularly significant given the pathway's influence on cellular proliferation and differentiation. ERK8's interaction with these pathways emphasizes its contribution to controlling cellular responses and adapting to environmental changes.
- Associated diseases and disorders
ERK8's functions relate to cancer and metabolic disorders. Its aberrant activity is associated with tumor progression as it can influence processes like cell proliferation and evasion of apoptosis. In the context of cancer ERK8 can interact with proteins like c-Jun a component of the AP-1 transcription factor complex to promote oncogenic transformations. Additionally ERK8 is implicated in metabolic disorders through its role in regulating glucose metabolism and insulin signaling making it a potential target for therapeutic intervention in diseases like diabetes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ERK8 antibody (ab137619)
7.5% SDS PAGE
All lanes: Western blot - Anti-ERK8 antibody (ab137619) at 1/1000 dilution
All lanes: HeLaS3 whole cell lysate at 30 µg
Predicted band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ERK8 antibody (ab137619)
ab137619 at 1/200 dilution staining ERK8 in methanol fixed A431 cells by Immunofluorescence. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK8 antibody (ab137619)
Immunohistochemical analysis of Human lung tissue, staining ERK8 in cytoplasm with ab137619, at 1/500 dilution.
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