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AB250392

Anti-ERLEC1 antibody [EPR13849] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ERLEC1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C2orf30, XTP3TPB, UNQ1878/PRO4321, ERLEC1, Endoplasmic reticulum lectin 1, ER lectin, XTP3-transactivated gene B protein, Erlectin

2 Images
Western blot - Anti-ERLEC1 antibody [EPR13849] - BSA and Azide free (AB250392)
  • WB

Supplier Data

Western blot - Anti-ERLEC1 antibody [EPR13849] - BSA and Azide free (AB250392)

This data was developed using ab181166, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ERLEC1 antibody [EPR13849] (<a href='/en-us/products/primary-antibodies/erlec1-antibody-epr13849-ab181166'>ab181166</a>) at 1/2000 dilution

All lanes:

A375 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Western blot - Anti-ERLEC1 antibody [EPR13849] - BSA and Azide free (AB250392)
  • WB

Supplier Data

Western blot - Anti-ERLEC1 antibody [EPR13849] - BSA and Azide free (AB250392)

This data was developed using ab181166, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ERLEC1 antibody [EPR13849] (<a href='/en-us/products/primary-antibodies/erlec1-antibody-epr13849-ab181166'>ab181166</a>) at 1/10000 dilution

All lanes:

HepG2 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13849

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250392 is the carrier-free version of ab181166.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERLEC1 also known as Endoplasmic Reticulum Lectin 1 or XTP3-B is a protein involved in maintaining protein quality control in the endoplasmic reticulum (ER). ERLEC1 has a molecular mass of about 38 kDa. The protein localizes in the ER where it participates in the calnexin/calreticulin cycle and binds to misfolded glycoproteins. Its role as a lectin allows it to recognize specific glycan structures on proteins aiding in their proper folding and maturation.
Biological function summary

ERLEC1 contributes to the ER-associated degradation (ERAD) pathway by interacting with other ER chaperones and lectin-like proteins. ERLEC1 forms part of a protein complex that identifies misfolded glycoproteins and targets them for degradation. Its function is important for relieving the ER of protein overload therefore maintaining cellular homeostasis. This role is particularly significant in tissues with high protein synthesis rates such as the liver and pancreas.

Pathways

ERLEC1 plays an important role in the unfolded protein response (UPR) by assisting in the degradation of malfunctioning proteins. It interacts with other proteins such as HRD1 and SEL1L which are part of the ERAD pathway to achieve this function. ERLEC1 contributes to maintaining cellular health by ensuring only correctly folded proteins proceed to their destination therefore preventing stress induced by protein aggregation.

ERLEC1's role in protein degradation links it to neurodegenerative diseases like Parkinson's and Alzheimer's. Misfolded protein accumulation which ERLEC1 helps prevent is a hallmark of these diseases. Disruption of ERLEC1 function may also relate to conditions like cystic fibrosis where improper protein folding and processing are involved. Protein connections to disorders include interactions with well-studied pathological proteins like alpha-synuclein and amyloid-beta highlighting its therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable lectin that binds selectively to improperly folded lumenal proteins. May function in endoplasmic reticulum quality control and endoplasmic reticulum-associated degradation (ERAD) of both non-glycosylated proteins and glycoproteins.
See full target information ERLEC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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