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AB129207

Anti-Erlin-2 antibody [EPR8088]

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(4 Publications)

Rabbit Recombinant Monoclonal Erlin-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

C8orf2, SPFH2, UNQ2441/PRO5003/PRO9924, ERLIN2, Erlin-2, Endoplasmic reticulum lipid raft-associated protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2, SPFH domain-containing protein 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8088] (AB129207)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8088] (AB129207)

ab129207 at 1/50 dilution staining Erlin-2 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Erlin-2 antibody [EPR8088] (AB129207)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Erlin-2 antibody [EPR8088] (AB129207)

ab129207 at 1/100 dilution staining Erlin-2 in HepG2 cells by Immunofluorescence.

Western blot - Anti-Erlin-2 antibody [EPR8088] (AB129207)
  • WB

Unknown

Western blot - Anti-Erlin-2 antibody [EPR8088] (AB129207)

All lanes:

Western blot - Anti-Erlin-2 antibody [EPR8088] (ab129207) at 1/1000 dilution

Lane 1:

Fetal kidney tissue lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 38 kDa

false

OI-RD Scanning - Anti-Erlin-2 antibody [EPR8088] (AB129207)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Erlin-2 antibody [EPR8088] (AB129207)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Erlin-2 antibody [EPR8088] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8088

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erlin-2 also known as SPFH domain family member 2 is a protein with an approximate mass of 40 kDa. The protein is expressed in the endoplasmic reticulum where it participates in various cellular processes. Erlin-2 shares similarities structural with its homolog Erlin-1. Erlin-2 is part of a larger family of erlins that play essential roles in cellular regulation.
Biological function summary

The functions of Erlin-2 involve its participation as a member of the erlins complex which includes Erlin-1 and other proteins. This complex helps regulate the degradation of inositol 145-trisphosphate receptors. Furthermore Erlin-2 facilitates the process of lipid raft-associated protein transport and maintains lipid homeostasis inside cells.

Pathways

Erlin-2 contributes to the endoplasmic reticulum-associated degradation pathway which is important for managing protein quality control. In coordination with Erlin-1 Erlin-2 influences calcium signaling pathways by regulating the turnover of inositol 145-trisphosphate receptors. These pathways play a significant role in maintaining cellular homeostasis and responding to stress conditions.

Researchers have linked Erlin-2 to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). Abnormalities in calcium signaling pathways mediated by Erlin-2 and associated with inositol 145-trisphosphate receptors can contribute to neuronal dysfunction in these diseases. Additionally changes in Erlin-2 expression may relate to certain cancers suggesting its potential role in tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed : 17502376, PubMed : 19240031). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed : 21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed : 24217618).
See full target information ERLIN2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 76:103326 PubMed39180984

2024

The activity of therapeutic molecular cluster Ag5 is dependent on oxygen level and HIF-1 mediated signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie A Twigger,Blanca Dominguez,Vanesa Porto,Lina Hacker,Anthony J Chalmers,Ross Breckenridge,Martin Treder,Adam C Sedgwick,Fernando Dominguez,Ester M Hammond

Cellular and molecular neurobiology 43:2219-2241 PubMed36571634

2022

Alzheimer's-Associated Upregulation of Mitochondria-Associated ER Membranes After Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Rishi R Agrawal,Delfina Larrea,Yimeng Xu,Lingyan Shi,Hylde Zirpoli,Leslie G Cummins,Valentina Emmanuele,Donghui Song,Taekyung D Yun,Frank P Macaluso,Wei Min,Steven G Kernie,Richard J Deckelbaum,Estela Area-Gomez

Cell reports 37:109932 PubMed34731613

2021

Quantal Ca release mediated by very few IP receptors that rapidly inactivate allows graded responses to IP.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Rossi,Andrew M Riley,Geneviève Dupont,Taufiq Rahman,Barry V L Potter,Colin W Taylor

Frontiers in immunology 11:568440 PubMed33424830

2020

Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Liu,Pengfei Xie,Daishang Jiang,Jian Liu,Jianguo Zhang,Tingting Bian,Jiahai Shi
View all publications
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