AB128924

Anti-Erlin-2 antibody [EPR8089]

Name: Anti-Erlin-2 antibody [EPR8089] (ab128924) | Abcam
Brand: Abcam Limited
SKU: AB128924
Price: 325 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal Erlin-2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

C8orf2, SPFH2, UNQ2441/PRO5003/PRO9924, ERLIN2, Erlin-2, Endoplasmic reticulum lipid raft-associated protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2, SPFH domain-containing protein 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] (AB128924)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] (AB128924)

ab128924 at 1/50 dilution staining Erlin-2 in paraffin-embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Erlin-2 antibody [EPR8089] (AB128924)

All lanes:

Western blot - Anti-Erlin-2 antibody [EPR8089] (ab128924) at 1/10000 dilution

Lane 1:

Fetal kidney lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

false

Carrier free

Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8089

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erlin-2 also known as SPFH domain family member 2 is a protein with an approximate mass of 40 kDa. The protein is expressed in the endoplasmic reticulum where it participates in various cellular processes. Erlin-2 shares similarities structural with its homolog Erlin-1. Erlin-2 is part of a larger family of erlins that play essential roles in cellular regulation.

Biological function summary

The functions of Erlin-2 involve its participation as a member of the erlins complex which includes Erlin-1 and other proteins. This complex helps regulate the degradation of inositol 145-trisphosphate receptors. Furthermore Erlin-2 facilitates the process of lipid raft-associated protein transport and maintains lipid homeostasis inside cells.

Pathways

Erlin-2 contributes to the endoplasmic reticulum-associated degradation pathway which is important for managing protein quality control. In coordination with Erlin-1 Erlin-2 influences calcium signaling pathways by regulating the turnover of inositol 145-trisphosphate receptors. These pathways play a significant role in maintaining cellular homeostasis and responding to stress conditions.

Researchers have linked Erlin-2 to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). Abnormalities in calcium signaling pathways mediated by Erlin-2 and associated with inositol 145-trisphosphate receptors can contribute to neuronal dysfunction in these diseases. Additionally changes in Erlin-2 expression may relate to certain cancers suggesting its potential role in tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed : 17502376, PubMed : 19240031). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed : 21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed : 24217618).

See full target information ERLIN2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 20:839 PubMed32883232

2020

SNHG17 drives malignant behaviors in astrocytoma by targeting miR-876-5p/ERLIN2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengping Du,Qian Hou

Molecular cell 79:768-781.e7 PubMed32738194

2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L van de Weijer,Logesvaran Krshnan,Sabrina Liberatori,Elena Navarro Guerrero,Jacob Robson-Tull,Lilli Hahn,Robert Jan Lebbink,Emmanuel J H J Wiertz,Roman Fischer,Daniel Ebner,Pedro Carvalho

Cell 171:179-187.e10 PubMed28890085

2017

In Situ Architecture and Cellular Interactions of PolyQ Inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Felix J B Bäuerlein,Itika Saha,Archana Mishra,Maria Kalemanov,Antonio Martínez-Sánchez,Rüdiger Klein,Irina Dudanova,Mark S Hipp,F Ulrich Hartl,Wolfgang Baumeister,Rubén Fernández-Busnadiego

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Erlin-2 antibody [EPR8089]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] (AB128924)

Western blot - Anti-Erlin-2 antibody [EPR8089] (AB128924)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise