Rabbit Recombinant Monoclonal Erlin-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (AB248223), expandable thumbnail](https://content.abcam.com/products/images/erlin-2-antibody-epr8089-bsa-and-azide-free-ab248223--western-blot-img126027.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (AB248223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (AB248223), expandable thumbnail](https://content.abcam.com/products/images/erlin-2-antibody-epr8089-bsa-and-azide-free-ab248223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135446.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (AB248223)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:17502376, PubMed:19240031). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618).
Alternative names
C8orf2, SPFH2, UNQ2441/PRO5003/PRO9924, ERLIN2, Erlin-2, Endoplasmic reticulum lipid raft-associated protein 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2, SPFH domain-containing protein 2
Recommended products
Rabbit Recombinant Monoclonal Erlin-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8089
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248223 is the carrier-free version of Anti-Erlin-2 antibody [EPR8089] ab128924.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Erlin-2 also known as SPFH domain family member 2 is a protein with an approximate mass of 40 kDa. The protein is expressed in the endoplasmic reticulum where it participates in various cellular processes. Erlin-2 shares similarities structural with its homolog Erlin-1. Erlin-2 is part of a larger family of erlins that play essential roles in cellular regulation.
- Biological function summary
The functions of Erlin-2 involve its participation as a member of the erlins complex which includes Erlin-1 and other proteins. This complex helps regulate the degradation of inositol 145-trisphosphate receptors. Furthermore Erlin-2 facilitates the process of lipid raft-associated protein transport and maintains lipid homeostasis inside cells.
- Pathways
Erlin-2 contributes to the endoplasmic reticulum-associated degradation pathway which is important for managing protein quality control. In coordination with Erlin-1 Erlin-2 influences calcium signaling pathways by regulating the turnover of inositol 145-trisphosphate receptors. These pathways play a significant role in maintaining cellular homeostasis and responding to stress conditions.
- Associated diseases and disorders
Researchers have linked Erlin-2 to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS). Abnormalities in calcium signaling pathways mediated by Erlin-2 and associated with inositol 145-trisphosphate receptors can contribute to neuronal dysfunction in these diseases. Additionally changes in Erlin-2 expression may relate to certain cancers suggesting its potential role in tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223), expandable thumbnail](https://content.abcam.com/products/images/erlin-2-antibody-epr8089-bsa-and-azide-free-ab248223--western-blot-img126027.jpg "Western blot - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223)")
Western blot - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223)
This data was developed using Anti-Erlin-2 antibody [EPR8089] ab128924, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Erlin-2 antibody [EPR8089] (Anti-Erlin-2 antibody [EPR8089] ab128924) at 1/10000 dilution
Lane 1: Fetal kidney lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223), expandable thumbnail](https://content.abcam.com/products/images/erlin-2-antibody-epr8089-bsa-and-azide-free-ab248223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135446.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erlin-2 antibody [EPR8089] - BSA and Azide free (ab248223)
This data was developed using Anti-Erlin-2 antibody [EPR8089] ab128924, the same antibody clone in a different buffer formulation.Anti-Erlin-2 antibody [EPR8089] ab128924 at 1/50 dilution staining Erlin-2 in paraffin-embedded Human colon tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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