Anti-ERM / Etv5 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERM / Etv5 antibody (AB102010)

ab102010 staining ERM / Etv5 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/500) and cytoskeleton stained red with Phalloidin.

• WB

Lab

Western blot - Anti-ERM / Etv5 antibody (AB102010)

Lanes 1 - 2 : Merged signal (red and green). Green - ab102010 observed at 58 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab102010 was shown to react with ERM / Etv5 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab102010 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution

Lane 1:

PC-3 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-ERM / Etv5 antibody (AB102010)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution

All lanes:

PC-3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-ERM / Etv5 antibody (AB102010)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-ERM / Etv5 antibody (AB102010)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution

All lanes:

NCI-H929 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-ERM / Etv5 antibody (AB102010)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ERM / Etv5 antibody (ab102010) at 1/5000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

Myc-DDK-tag tagged ERM transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false