Rabbit Recombinant Monoclonal ERO1L antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.
View Alternative Names
ERO1L, UNQ434/PRO865, ERO1A, ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic oxidoreductin-1-like protein, Endoplasmic reticulum oxidoreductase alpha, Oxidoreductin-1-L-alpha
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-ERO1L antibody [EPR12474] (AB177156)
Intracellular flow cytometric analysis of permeabilized HeLacells labeling ERO1Lwith ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1L antibody [EPR12474] (AB177156)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
- IP
Supplier Data
Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (AB177156)
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes:
Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (ab177156)
Predicted band size: 54 kDa
false
- WB
Supplier Data
Western blot - Anti-ERO1L antibody [EPR12474] (AB177156)
All lanes:
Western blot - Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilution
Lane 1:
HepG2 cell lysate at 10 µg
Lane 2:
293T cell lysate at 10 µg
Lane 3:
MCF-7 cell lysate at 10 µg
Lane 4:
HeLa cell lysate at 10 µg
Secondary
All lanes:
Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 54 kDa
false
Related conjugates and formulations (10)
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Anti-ERO1L antibody [EPR12474] - BSA and Azide free
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578 PE
PE Anti-ERO1L antibody [EPR12474]
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660 APC
APC Anti-ERO1L antibody [EPR12474]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-ERO1L antibody [EPR12474]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-ERO1L antibody [EPR12474]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-ERO1L antibody [EPR12474]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-ERO1L antibody [EPR12474]
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HRP Anti-ERO1L antibody [EPR12474]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-ERO1L antibody [EPR12474]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-ERO1L antibody [EPR12474]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ERO1L contributes to oxidative protein folding within the endoplasmic reticulum. As part of a complex it interacts with protein disulfide isomerases to facilitate the introduction of disulfide bonds into nascent proteins. This function is essential for the proper maturation of secreted proteins influencing cellular protein management processes. ERO1L activity impacts cellular responses to stress and influences the capacity of cells to handle unfolded proteins.
Pathways
ERO1L significantly influences the protein folding and endoplasmic reticulum-associated degradation pathway. It has strong associations with protein disulfide isomerase and interacts within the unfolded protein response to manage cellular stress caused by misfolded proteins. The protein contributes to the maintenance of a stable internal environment during conditions where protein folding demands increase.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (14)
Recent publications for all applications. Explore the full list and refine your search
iScience 28:112748 PubMed40585507
2025
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APL bioengineering 8:046114 PubMed39691350
2024
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Unspecified reactive species
Cell reports. Medicine 4:101206 PubMed37769655
2023
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Unspecified reactive species
Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449
2023
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Unspecified application
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Unspecified reactive species
Journal of translational medicine 20:462 PubMed36209225
2022
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Unspecified reactive species
Bioengineered 13:10061-10070 PubMed35441565
2022
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Unspecified reactive species
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:823-834 PubMed33687617
2021
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Unspecified application
Species
Unspecified reactive species
Journal of immunology research 2021:5553425 PubMed33681386
2021
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Unspecified reactive species
Frontiers in immunology 11:609337 PubMed33569057
2021
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Unspecified reactive species
Molecular & cellular proteomics : MCP 20:100015 PubMed33508502
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com