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AB177156

Anti-ERO1L antibody [EPR12474]

5

(2 Reviews)

|

(14 Publications)

Rabbit Recombinant Monoclonal ERO1L antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications.

View Alternative Names

ERO1L, UNQ434/PRO865, ERO1A, ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic oxidoreductin-1-like protein, Endoplasmic reticulum oxidoreductase alpha, Oxidoreductin-1-L-alpha

4 Images
Flow Cytometry (Intracellular) - Anti-ERO1L antibody [EPR12474] (AB177156)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERO1L antibody [EPR12474] (AB177156)

Intracellular flow cytometric analysis of permeabilized HeLacells labeling ERO1Lwith ab177156 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1L antibody [EPR12474] (AB177156)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1L antibody [EPR12474] (AB177156)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ERO1L with ab177156 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (AB177156)
  • IP

Supplier Data

Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (AB177156)

Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1 X PBS (negative control) using ab177156 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-ERO1L antibody [EPR12474] (ab177156)

Predicted band size: 54 kDa

false

Western blot - Anti-ERO1L antibody [EPR12474] (AB177156)
  • WB

Supplier Data

Western blot - Anti-ERO1L antibody [EPR12474] (AB177156)

All lanes:

Western blot - Anti-ERO1L antibody [EPR12474] (ab177156) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 54 kDa

false

  • Carrier free

    Anti-ERO1L antibody [EPR12474] - BSA and Azide free

  • 578 PE

    PE Anti-ERO1L antibody [EPR12474]

  • 660 APC

    APC Anti-ERO1L antibody [EPR12474]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-ERO1L antibody [EPR12474]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ERO1L antibody [EPR12474]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ERO1L antibody [EPR12474]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ERO1L antibody [EPR12474]

  • HRP

    HRP Anti-ERO1L antibody [EPR12474]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-ERO1L antibody [EPR12474]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-ERO1L antibody [EPR12474]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12474

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERO1L also known as ERO1-alpha is a protein involved in the formation of disulfide bonds in the endoplasmic reticulum important for protein folding. The molecular mass of ERO1L is approximately 54 kDa. ERO1L primarily expresses in tissues with high secretory activities such as the liver and pancreas. It plays a vital role in maintaining redox homeostasis within the cell by transferring electrons from protein disulfide isomerase to molecular oxygen.
Biological function summary

ERO1L contributes to oxidative protein folding within the endoplasmic reticulum. As part of a complex it interacts with protein disulfide isomerases to facilitate the introduction of disulfide bonds into nascent proteins. This function is essential for the proper maturation of secreted proteins influencing cellular protein management processes. ERO1L activity impacts cellular responses to stress and influences the capacity of cells to handle unfolded proteins.

Pathways

ERO1L significantly influences the protein folding and endoplasmic reticulum-associated degradation pathway. It has strong associations with protein disulfide isomerase and interacts within the unfolded protein response to manage cellular stress caused by misfolded proteins. The protein contributes to the maintenance of a stable internal environment during conditions where protein folding demands increase.

ERO1L shows connections to cancer and neurodegenerative disorders. Aberrant expression of ERO1L can influence tumor growth due to its role in modulating cellular stress responses and managing protein folding in cancer cells. The protein also shows a relationship with neurodegenerative conditions through its involvement in protein misfolding pathways which are critical in diseases like Alzheimer's. ERO1L collaborates with proteins like protein disulfide isomerase and participates in cellular mechanisms underlying these conditions.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins (PubMed : 29858230). Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
See full target information ERO1A

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112748 PubMed40585507

2025

CETSA-MS unveils novel targets engaged by rigosertib to promote anti-tumor activity and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Petros Kechagioglou,Hajime Yurugi,Camille Dupont,Alexey Chernobrovkin,Rossana Romero,Gregory Harms,Marie Oster,Sandra Ciesek,Rebecca Tweedell,Thirumala-Devi Kanneganti,Stefanie Zimmer,Stephen Cosenza,Steven M Fruchtman,Krishnaraj Rajalingam

APL bioengineering 8:046114 PubMed39691350

2024

Immunogenic cell death-related cancer-associated fibroblast clusters and prognostic risk model in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wu,Yue Xu

Cell reports. Medicine 4:101206 PubMed37769655

2023

Ablation of ERO1A induces lethal endoplasmic reticulum stress responses and immunogenic cell death to activate anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Liu,Sini Li,Yan Qu,Hua Bai,Xiangyu Pan,Jian Wang,Zhijie Wang,Jianchun Duan,Jia Zhong,Rui Wan,Kailun Fei,Jiachen Xu,Li Yuan,Chao Wang,Pei Xue,Xue Zhang,Zixiao Ma,Jie Wang

Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449

2023

Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Ruijiang Lin,Minjie Ma,Biao Han,Ya Zheng,Yuping Wang,Yongning Zhou

Journal of translational medicine 20:462 PubMed36209225

2022

Decoding the colorectal cancer ecosystem emphasizes the cooperative role of cancer cells, TAMs and CAFsin tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Rongfang Shen,Ping Li,Botao Zhang,Lin Feng,Shujun Cheng

Bioengineered 13:10061-10070 PubMed35441565

2022

MicroRNA-218-5p affects lung adenocarcinoma progression through targeting endoplasmic reticulum oxidoreductase 1 alpha.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Chen,Qihao Wang,Kunyu Wang

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:823-834 PubMed33687617

2021

Tumor mutation burden as a biomarker in resected gastric cancer via its association with immune infiltration and hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Deqiang Wang,Ning Wang,Xiaoqin Li,Xiaofeng Chen,Bo Shen,Dongqin Zhu,Liuqing Zhu,Yaping Xu,Yangyang Yu,Yongqian Shu

Journal of immunology research 2021:5553425 PubMed33681386

2021

ERO1L Promotes Hepatic Metastasis through Activating Epithelial-Mesenchymal Transition (EMT) in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

JianYu Yang,Yu Xu,YanMiao Huo,Li Cai,Rong Hua,JunFeng Zhang,Zhong Chen

Frontiers in immunology 11:609337 PubMed33569057

2021

Tumor Microenvironment Status Predicts the Efficacy of Postoperative Chemotherapy or Radiochemotherapy in Resected Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Duan,Xiaoqin Li,Dongqiang Zeng,Xiaofeng Chen,Bo Shen,Dongqin Zhu,Liuqing Zhu,Yangyang Yu,Deqiang Wang

Molecular & cellular proteomics : MCP 20:100015 PubMed33508502

2021

Proteomic Analyses Identify Differentially Expressed Proteins and Pathways Between Low-Risk and High-Risk Subtypes of Early-Stage Lung Adenocarcinoma and Their Prognostic Impacts.

Applications

Unspecified application

Species

Unspecified reactive species

Juntuo Zhou,Bing Liu,Zhongwu Li,Yang Li,Xi Chen,Yuanyuan Ma,Shi Yan,Xin Yang,Lijun Zhong,Nan Wu
View all publications

Product promise

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