Anti-ERO1L antibody [RP23040237]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERO1L antibody [RP23040237] (AB313495)

Immunofluorescent analysis of DU145 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous ERO1L with ab313495 at 2 µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Ero1/endoplasmic oxidoreductin-1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of ERO1L. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-ERO1L Antibody [RP23040237] (AB313495)

All lanes:

Western blot - Anti-ERO1L Antibody [RP23040237] (ab313495) at 1 µg/mL

Lane 1:

SW480 cell lysate at 30 µg

Lane 2:

MCF7 cell lysate at 30 µg

Lane 3:

MDA-MB-231 cell lysate at 30 µg

Lane 4:

T47D cell lysate at 30 µg

Lane 5:

HCT116 cell lysate at 30 µg

Lane 6:

mouse placenta tissue lysate at 30 µg

Lane 7:

mouse ovary tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 54 kDa

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