Rabbit Polyclonal ERO1LB antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERO1B aa 250-450.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ERO1B aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YB8
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.
Alternative names
ERO1LB, ERO1B, ERO1-like protein beta, ERO1-L-beta, Endoplasmic reticulum oxidoreductase beta, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta
Recommended products
Rabbit Polyclonal ERO1LB antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ERO1B aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ERO1B aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YB8
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Add 200 µL of sterile distilled water.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERO1LB also known as Endoplasmic Reticulum Oxidoreductin 1 Beta or ERO1-like protein beta is an enzyme involved in oxidative protein folding. It is approximately 55 kDa in size. ERO1LB localizes mainly in the endoplasmic reticulum and is expressed in various tissues with higher expression in the pancreas and secretory tissues. This protein functions by reoxidizing PDI (protein disulfide isomerase) enzymes which assists in forming disulfide bonds essential for protein folding.
- Biological function summary
ERO1LB plays a role in the cellular response to oxidative stress and regulation of the redox state. It acts as part of a complex that maintains the proper folding of secretory and membrane proteins. ERO1LB helps maintain the balance between reducing equivalents in the endoplasmic reticulum and cellular homeostasis. It contributes to efficient protein secretion which is critical for tissues with high secretory demands.
- Pathways
ERO1LB is integral to the protein folding and secretory pathways. It participates in the oxidative folding pathway by regenerating the oxidized state of PDIs which directly impacts protein disulfide formation. ERO1LB interacts with proteins such as PDI and ERO1A to maintain redox homeostasis ensuring the efficacy of the protein folding machinery. It helps regulate the unfolded protein response (UPR) a pathway important for preventing the accumulation of misfolded proteins.
- Associated diseases and disorders
Oxidative stress-related conditions and pancreatic diseases involve the ERO1LB protein. Abnormal expression of ERO1LB can contribute to disorders like diabetes given its role in insulin biosynthesis. The protein interacts with insulin processing and secretion where a misbalance might result in disease. Additionally ERO1LB connects to oxidative stress mechanisms linking to the pathology of neurodegenerative diseases through shared pathways with other oxidoreductases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ERO1LB antibody (ab197290)
All lanes: Western blot - Anti-ERO1LB antibody (ab197290) at 1/500 dilution
All lanes: human fetal testis lysate
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody (ab197290)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human fetal testis tissue labeling ERO1LB with ab197290 at a 1/100 dilution.
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