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AB230540

Anti-ERO1LB antibody - C-terminal

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Rabbit Polyclonal ERO1LB antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ERO1B aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ERO1LB, ERO1B, ERO1-like protein beta, ERO1-L-beta, Endoplasmic reticulum oxidoreductase beta, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta

3 Images
Flow Cytometry (Intracellular) - Anti-ERO1LB antibody - C-terminal (AB230540)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERO1LB antibody - C-terminal (AB230540)

Intracellular flow cytometric analysis of MDA-MB-435 cell line labeling ERO1LB with ab230540 at 1/10 dilution (green) compared with a negative control (blue).

Western blot - Anti-ERO1LB antibody - C-terminal (AB230540)
  • WB

Supplier Data

Western blot - Anti-ERO1LB antibody - C-terminal (AB230540)

All lanes:

Western blot - Anti-ERO1LB antibody - C-terminal (ab230540) at 1/1000 dilution

All lanes:

MDA-MB435 whole cell lysate at 35 µg

Predicted band size: 54 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody - C-terminal (AB230540)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody - C-terminal (AB230540)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ERO1LB using ab230540 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ERO1B aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q86YB8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERO1LB also known as Endoplasmic Reticulum Oxidoreductin 1 Beta or ERO1-like protein beta is an enzyme involved in oxidative protein folding. It is approximately 55 kDa in size. ERO1LB localizes mainly in the endoplasmic reticulum and is expressed in various tissues with higher expression in the pancreas and secretory tissues. This protein functions by reoxidizing PDI (protein disulfide isomerase) enzymes which assists in forming disulfide bonds essential for protein folding.
Biological function summary

ERO1LB plays a role in the cellular response to oxidative stress and regulation of the redox state. It acts as part of a complex that maintains the proper folding of secretory and membrane proteins. ERO1LB helps maintain the balance between reducing equivalents in the endoplasmic reticulum and cellular homeostasis. It contributes to efficient protein secretion which is critical for tissues with high secretory demands.

Pathways

ERO1LB is integral to the protein folding and secretory pathways. It participates in the oxidative folding pathway by regenerating the oxidized state of PDIs which directly impacts protein disulfide formation. ERO1LB interacts with proteins such as PDI and ERO1A to maintain redox homeostasis ensuring the efficacy of the protein folding machinery. It helps regulate the unfolded protein response (UPR) a pathway important for preventing the accumulation of misfolded proteins.

Oxidative stress-related conditions and pancreatic diseases involve the ERO1LB protein. Abnormal expression of ERO1LB can contribute to disorders like diabetes given its role in insulin biosynthesis. The protein interacts with insulin processing and secretion where a misbalance might result in disease. Additionally ERO1LB connects to oxidative stress mechanisms linking to the pathology of neurodegenerative diseases through shared pathways with other oxidoreductases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.
See full target information ERO1B
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