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Knockout Tested Rabbit Recombinant Monoclonal Eros antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Eros antibody [EPR28697-6] (AB316209), expandable thumbnail
  • Western blot - Anti-Eros antibody [EPR28697-6] (AB316209), expandable thumbnail
  • Western blot - Anti-Eros antibody [EPR28697-6] (AB316209), expandable thumbnail
  • Western blot - Anti-Eros antibody [EPR28697-6] (AB316209), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)ICC/IFIHC-PIP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Functions as a chaperone necessary for a stable expression of the CYBA and CYBB subunits of the cytochrome b-245 heterodimer (PubMed:30361506). Controls the phagocyte respiratory burst and is essential for innate immunity (By similarity).

Alternative names

C17orf62, EROS, CYBC1, Cytochrome b-245 chaperone 1, Essential for reactive oxygen species protein, Eros

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Eros antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28697-6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The biological target known as Eros also referred to as 'peptides’ or 'anti Eros' is a protein associated with various cellular functions. Eros has a molecular mass typically around 30 kDa. It is expressed in multiple tissues prominently in the cells of the immune system. This protein modulates immune responses and cell signaling. The distribution of Eros is essential for effective cellular defense mechanisms.

Biological function summary

The function of Eros extends beyond simple protein interactions. It contributes significantly to reactive oxygen species (ROS) production which is important in phagocytic processes. Eros acts as part of the NADPH oxidase complex a multi-component enzyme system found within phagocytes. The assembly of this complex remains fundamental for generating ROS facilitating microbial killing during immune defenses and inflammation.

Pathways

Eros operates intricately within the immune response modulation and redox regulation pathways. It plays an important role in the oxidative burst pathway where its presence is necessary for proper NADPH oxidase function. This involves interactions with protein components such as gp91phox and p22phox which are part of the membrane-bound cytochrome b558. Eros helps to maintain optimal activity of this complex ensuring effective pathogen destruction.

Associated diseases and disorders

Eros is linked with chronic granulomatous disease (CGD) and inflammatory response dysregulation. Deficient Eros function can lead to CGD a condition caused by an inability to produce sufficient ROS for microbial destruction. This connection highlights the relationships between Eros and other components of the NADPH oxidase complex including p47phox which also suffers mutations in CGD. Dysfunctional Eros may also contribute to inflammatory disorders potentially influencing disease progression and severity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Eros antibody [EPR28697-6] (ab316209), expandable thumbnail

    Western blot - Anti-Eros antibody [EPR28697-6] (ab316209)

    Western blot: Anti-CYBC1 antibody [EPR28697-6] (ab316209) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab316209 was shown to bind specifically to CYBC1. A band was observed at 37 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in CYBC1 knockout cell line. To generate this image, wild-type and CYBC1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

    Lane 1: Wild-type HCT 116 cell lysate at 20 µg

    Lane 2: CYBC1 knockout HCT 116 cell lysate at 20 µg

    Lane 3: THP-1 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Lane 5: HEK-293 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa

  • Western blot - Anti-Eros antibody [EPR28697-6] (ab316209), expandable thumbnail

    Western blot - Anti-Eros antibody [EPR28697-6] (ab316209)

    Low expression: skeletal muscle, heart

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

    Lane 1: Human spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human lymph node tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Mouse lymph node tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 7: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 8: Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Eros antibody [EPR28697-6] (ab316209), expandable thumbnail

    Western blot - Anti-Eros antibody [EPR28697-6] (ab316209)

    All lanes: Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa

    Exposure time: 180s

  • Western blot - Anti-Eros antibody [EPR28697-6] (ab316209), expandable thumbnail

    Western blot - Anti-Eros antibody [EPR28697-6] (ab316209)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane1-2: 180 seconds, lane3: 59 seconds, lane4-5: 180 seconds

    All lanes: Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: PC-3 transfected with siRNA specifically targeting Eros whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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