Anti-Eros antibody [EPR28697-6]

• WB

Lab

Western blot - Anti-Eros antibody [EPR28697-6] (AB316209)

Western blot : Anti-CYBC1 antibody [EPR28697-6] (ab316209) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab316209 was shown to bind specifically to CYBC1. A band was observed at 18 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in CYBC1 knockout cell line. To generate this image, wild-type and CYBC1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

CYBC1 knockout HCT 116 cell lysate at 20 µg

Lane 3:

THP-1 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Lane 5:

HEK-293 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 18 kDa

false

• WB

Supplier Data

Western blot - Anti-Eros antibody [EPR28697-6] (AB316209)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-2 : 180 seconds, lane3 : 59 seconds, lane4-5 : 180 seconds

All lanes:

Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

PC-3 transfected with siRNA specifically targeting Eros whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Eros antibody [EPR28697-6] (AB316209)

Low expression : skeletal muscle, heart

The identity of the higher MW band at approximately 70 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Human lymph node tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Mouse lymph node tissue lysate at 20 µg with 5% NFDM/TBST

Lane 6:

Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST

Lane 7:

Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

Lane 8:

Rat skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Eros antibody [EPR28697-6] (AB316209)

All lanes:

Western blot - Anti-Eros antibody [EPR28697-6] (ab316209) at 1/1000 dilution

Lane 1:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

NR8383 (rat alveolar macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa

false

Exposure time: 180s