AB134938

Anti-ERp18 antibody [EPR9024]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal ERp18 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

TLP19, UNQ713/PRO1376, TXNDC12, Thioredoxin domain-containing protein 12, Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, Thioredoxin-like protein p19, hTLP19, ER protein 18, ERp18, ER protein 19, ERp19

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ERp18 antibody [EPR9024] (AB134938)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERp18 antibody [EPR9024] (AB134938)

Immunofluorescence analysis of HepG2 cells labelling ERp18 using ab134938 at a 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp18 antibody [EPR9024] (AB134938)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp18 antibody [EPR9024] (AB134938)

Immunohistochemical analysis of paraffin-embedded formalin-fixed Human kidney tissue labelling ERp18 using ab134938 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ERp18 antibody [EPR9024] (AB134938)

All lanes:

Western blot - Anti-ERp18 antibody [EPR9024] (ab134938) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

true

Carrier free

Anti-ERp18 antibody [EPR9024] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9024

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp18 also known as PDIA6 or Protein Disulfide Isomerase Family A Member 6 is an endoplasmic reticulum (ER) resident protein with a molecular mass of approximately 18 kDa. This protein is known to act as a chaperone and assists in protein folding by catalyzing disulfide bond formation. ERp18 is widely expressed in various tissues particularly in those with high protein synthesis demands such as the liver and pancreas. It localizes mainly within the lumen of the ER.

Biological function summary

ERp18 plays a role in maintaining protein homeostasis. As a member of the protein disulfide isomerase (PDI) family it is involved in the formation and rearrangement of disulfide bonds essential for proper protein architecture. ERp18 functions collaboratively with other PDIs in the ER suggesting it may be part of a larger protein complex. The efficient folding and structural stabilization of proteins are critical for cellular function and survival.

Pathways

ERp18 contributes significantly to the protein-folding pathway and is linked to the unfolded protein response (UPR). The protein is instrumental in mitigating ER stress conditions which arise when there is an accumulation of misfolded proteins. By interacting with other PDI family proteins like PDI itself and ERp72 ERp18 ensures proteins achieve correct configurations which is necessary for maintaining cellular equilibrium and preventing apoptosis.

The malfunction or dysregulation of ERp18 associates with certain neurodegenerative diseases such as Alzheimer's disease where protein misfolding plays an important role. Moreover it has connections to cancer where the proliferation of cells often correlates with increased protein synthesis and folding demands. ERp18 interacts with proteins involved in these diseases like amyloid precursor protein (APP) in Alzheimer's and HIF-1α in cancer highlighting its importance in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein-disulfide reductase of the endoplasmic reticulum that promotes disulfide bond formation in client proteins through its thiol-disulfide oxidase activity.

See full target information TXNDC12

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Research and practice in thrombosis and haemostasis 9:102706 PubMed40224275

2025

Thiol isomerase ERp18 enhances platelet activation and arterial thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao He,Aizhen Yang,Keyu Lv,Yuxin Zhang,Zhenzhen Zhao,Yi Lu,Chao Fang,Yue Han,Depei Wu,Miao Jiang,Jingyu Zhang,Yi Wu

Thrombosis journal 22:110 PubMed39696500

2024

A novel role for protein disulfide isomerase ERp18 in venous thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao He,Aizhen Yang,Yuxin Zhang,Zhenzhen Zhao,Yi Lu,Jingyu Zhang,Yi Wu

Journal of Cancer 15:3913-3929 PubMed38911386

2024

TXNDC12 inhibits pancreatic tumor cells ferroptosis by regulating GSH/GGT7 and promotes its growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrong Xu,Yu Hei,Bobo Wang,Shuyue Tian,Xuanyu Chen,Jing Zhang,Fenghui Wang

The Journal of biological chemistry 298:102677 PubMed36336075

2022

A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yonatan G Mideksa,Isabel Aschenbrenner,Anja Fux,Dinah Kaylani,Caroline A M Weiß,Tuan-Anh Nguyen,Nina C Bach,Kathrin Lang,Stephan A Sieber,Matthias J Feige

Journal of oncology 2022:4488640 PubMed35836489

2022

PLCD1-Induced DNA Damage Inhibits the Tumor Growth via Downregulating CDKs in Chondrosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiakang Shen,Chen Yu,Zhuoying Wang,Haoran Mu,Zhengdong Cai

EMBO reports 22:e51412 PubMed33710763

2021

Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Sicari,Federica G Centonze,Raphael Pineau,Pierre-Jean Le Reste,Luc Negroni,Sophie Chat,M Aiman Mohtar,Daniel Thomas,Reynald Gillet,Ted Hupp,Eric Chevet,Aeid Igbaria

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp18 antibody [EPR9024]

Immunocytochemistry/ Immunofluorescence - Anti-ERp18 antibody [EPR9024] (AB134938)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp18 antibody [EPR9024] (AB134938)

Western blot - Anti-ERp18 antibody [EPR9024] (AB134938)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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