AB137035

Anti-ERp18 antibody [EPR9025]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ERp18 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TLP19, UNQ713/PRO1376, TXNDC12, Thioredoxin domain-containing protein 12, Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, Thioredoxin-like protein p19, hTLP19, ER protein 18, ERp18, ER protein 19, ERp19

2 Images

Flow Cytometry (Intracellular) - Anti-ERp18 antibody [EPR9025] (AB137035)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ERp18 antibody [EPR9025] (AB137035)

Intracellular Flow Cytometry analysis of permeabilized 293T cells labelling ERp18 with ab137035 at 1/10 dilution (Red) or a Rabbit IgG (negative control) (Green).

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Western blot - Anti-ERp18 antibody [EPR9025] (AB137035)

All lanes:

Western blot - Anti-ERp18 antibody [EPR9025] (ab137035) at 1/10000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

Y-79 cell lysate at 10 µg

Lane 4:

Human fetal kidney lysates at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 19 kDa

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Carrier free

Anti-ERp18 antibody [EPR9025] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9025

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp18 also known as PDIA6 or Protein Disulfide Isomerase Family A Member 6 is an endoplasmic reticulum (ER) resident protein with a molecular mass of approximately 18 kDa. This protein is known to act as a chaperone and assists in protein folding by catalyzing disulfide bond formation. ERp18 is widely expressed in various tissues particularly in those with high protein synthesis demands such as the liver and pancreas. It localizes mainly within the lumen of the ER.

Biological function summary

ERp18 plays a role in maintaining protein homeostasis. As a member of the protein disulfide isomerase (PDI) family it is involved in the formation and rearrangement of disulfide bonds essential for proper protein architecture. ERp18 functions collaboratively with other PDIs in the ER suggesting it may be part of a larger protein complex. The efficient folding and structural stabilization of proteins are critical for cellular function and survival.

Pathways

ERp18 contributes significantly to the protein-folding pathway and is linked to the unfolded protein response (UPR). The protein is instrumental in mitigating ER stress conditions which arise when there is an accumulation of misfolded proteins. By interacting with other PDI family proteins like PDI itself and ERp72 ERp18 ensures proteins achieve correct configurations which is necessary for maintaining cellular equilibrium and preventing apoptosis.

The malfunction or dysregulation of ERp18 associates with certain neurodegenerative diseases such as Alzheimer's disease where protein misfolding plays an important role. Moreover it has connections to cancer where the proliferation of cells often correlates with increased protein synthesis and folding demands. ERp18 interacts with proteins involved in these diseases like amyloid precursor protein (APP) in Alzheimer's and HIF-1α in cancer highlighting its importance in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein-disulfide reductase of the endoplasmic reticulum that promotes disulfide bond formation in client proteins through its thiol-disulfide oxidase activity.

See full target information TXNDC12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105538 PubMed38072046

2023

Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.

Applications

Unspecified application

Species

Unspecified reactive species

Peijun Wang,Na Fan,Wanting Yang,Pengbo Cao,Guojun Liu,Qi Zhao,Pengfei Guo,Xihe Li,Xinhua Lin,Ning Jiang,Buhe Nashun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp18 antibody [EPR9025]

Flow Cytometry (Intracellular) - Anti-ERp18 antibody [EPR9025] (AB137035)

Western blot - Anti-ERp18 antibody [EPR9025] (AB137035)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Proteins & Peptides

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise