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AB181172

Anti-ERP27 antibody [EPR12503]

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(3 Publications )

Rabbit Recombinant Monoclonal ERP27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 3 publications.

View Alternative Names

C12orf46, UNQ781/PRO1575, ERP27, Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, Inactive protein disulfide-isomerase 27

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] (AB181172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] (AB181172)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ERP27 antibody [EPR12503] (AB181172)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERP27 antibody [EPR12503] (AB181172)

Intracellular Flow Cytometry analysis of2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using aGoat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] (AB181172)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] (AB181172)

Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

Western blot - Anti-ERP27 antibody [EPR12503] (AB181172)
  • WB

Lab

Western blot - Anti-ERP27 antibody [EPR12503] (AB181172)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602).

ab181172 specifically detects a band only in pancreas tissue and not in other tissues.

The larger band size observed in mouse and rat tissues compared to human tissue may be due to ERP having only one glycosylation site in humans versus two in mouse and rat.

All lanes:

Western blot - Anti-ERP27 antibody [EPR12503] (ab181172) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat pancreas tissue lysate at 20 µg

Lane 7:

Rat heart tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30-35 kDa

false

Exposure time: 7s

  • Carrier free

    Anti-ERP27 antibody [EPR12503] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12503

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>AB181172 specifically detects a band only in pancreas tissue and not in other tissues. &nbsp;The larger band size observed in mouse and rat tissues compared to human tissue may be due to ERP having only one glycosylation site in humans versus two in mouse and rat.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERP27 also known as Endoplasmic Reticulum Protein 27 is a protein component with a molecular mass of approximately 27 kDa. It resides within the endoplasmic reticulum (ER) of the cell where it participates in protein folding and processing. Expressed in many tissues ERP27 is part of the protein disulfide isomerase (PDI) family. Although its structural features do not include the typical thioredoxin-like active sites ERP27 influences protein maturation by assisting in the correct assembly and folding of nascent proteins.
Biological function summary

The protein exhibits important roles in the cellular stress response and quality control mechanisms within the endoplasmic reticulum. It does not form a stable complex with other proteins but interacts transiently to exert its function. ERP27 collaborates closely with other ER-resident proteins like ERP44 and the PDI family to enhance the checking and refolding of misfolded proteins maintaining cellular homeostasis.

Pathways

ERP27 participates directly in the unfolded protein response (UPR) pathway a critical mechanism activated during ER stress. This pathway involves ERP27's transient interactions within a network of proteins including ER chaperones and other PDIs to alleviate stress and restore normal function. Furthermore ERP27 plays a role in calcium homeostasis through these pathways interacting with proteins such as calnexin and calreticulin.

ERP27's impairment or dysregulation associates with neurodegenerative diseases like Alzheimer's and Parkinson’s. The protein's involvement in the UPR links it to cellular processes that are disrupted in these conditions. Moreover it connects to proteins such as amyloid precursor protein (APP) in Alzheimer's influencing amyloid-beta peptide production. In Parkinson’s interactions with parkin and related proteins highlight ERP27's potential implications in disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically binds unfolded proteins and may recruit protein disulfide isomerase PDIA3 to unfolded substrates (PubMed : 16940051, PubMed : 23192347). Binds protein substrates via a hydrophobic pocket in the C-terminal domain (PubMed : 16940051, PubMed : 23192347). May play a role in the unfolded stress response (PubMed : 23192347).
See full target information ERP27
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38912589

2024

Protein disulfide isomerase is essential for spermatogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiong Zhang,Aizhen Yang,Zhenzhen Zhao,Fengwu Chen,Xiaofeng Yan,Yue Han,Depei Wu,Yi Wu

Frontiers in oncology 13:1178595 PubMed37313465

2023

Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Fan,Jiajia Wang,Ruolei Li,Kexin Chang,Liuyin Liu,Yaping Wang,Zhe Wang,Bo Zhang,Cheng Ji,Jian Zhang,Suning Chen,Rui Ling

RNA (New York, N.Y.) 25:1731-1750 PubMed31511320

2019

HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Neckles,Robert E Boer,Nicholas Aboreden,Allison M Cross,Robert L Walker,Bong-Hyun Kim,Suntae Kim,John S Schneekloth,Natasha J Caplen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com