Anti-ERP27 antibody [EPR12503] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERP27 antibody [EPR12503] - BSA and Azide free (AB250398)

This data was developed using ab181172, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERP27 antibody [EPR12503] - BSA and Azide free (AB250398)

This data was developed using ab181172, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERP27 antibody [EPR12503] - BSA and Azide free (AB250398)

This data was developed using ab181172, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells, labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.

• WB

Lab

Western blot - Anti-ERP27 antibody [EPR12503] - BSA and Azide free (AB250398)

This data was developed using ab181172, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602).

ab181172 specifically detects a band only in pancreas tissue and not in other tissues.

The larger band size observed in mouse and rat tissues compared to human tissue may be due to ERP having only one glycosylation site in humans versus two in mouse and rat.

All lanes:

Western blot - Anti-ERP27 antibody [EPR12503] (<a href='/en-us/products/primary-antibodies/erp27-antibody-epr12503-ab181172'>ab181172</a>) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Rat pancreas tissue lysate at 20 µg

Lane 7:

Rat heart tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30-35 kDa

false

Exposure time: 7s