AB11420

Anti-ERp29 antibody

Name: Anti-ERp29 antibody (ab11420) | Abcam
Brand: Abcam Limited
SKU: AB11420
Rating: 5 (4 reviews)

(4 Reviews)

(19 Publications)

Rabbit Polyclonal ERP29 antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse, Rat, Human, Guinea pig, Hamster, Primates, Dog, Cow samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Erp29.

View Alternative Names

Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 31, ERp31

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.

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Western blot - Anti-ERp29 antibody (AB11420)

Western blot of ERp29 in HEK cell lysate using ab11420.

All lanes:

Western blot - Anti-ERp29 antibody (ab11420)

Predicted band size: 29 kDa

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Supplier Data

Western blot - Anti-ERp29 antibody (AB11420)

12% SDS-PAGE

All lanes:

Western blot - Anti-ERp29 antibody (ab11420) at 1 µg/mL

Lane 1:

MDCK (canine kidney cell line) membrane-enriched extract at 30 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) membrane-enriched extract at 30 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) membrane-enriched extract at 30 µg

Lane 4:

A549 (human lung carcinoma cell line) membrane-enriched extract at 30 µg

Lane 5:

MCF7 (human breast adenocarcinoma cell line) membrane-enriched extract at 30 µg

Lane 6:

A431 (human epidermoid carcinoma cell line) membrane-enriched extract at 30 µg

Lane 7:

HEK-293 (human epithelial cell line from embryonic kidney) membrane-enriched extract at 30 µg

Predicted band size: 29 kDa

true

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog, Human, Primates, Hamster, Cow, Guinea pig

Applications

Flow Cyt, ICC/IF, WB, IP, IHC-Fr

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Erp29.

P52555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Hamster": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Primates": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2500", "WB-species-notes": "This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp29 also known as Endoplasmic Reticulum Resident Protein 29 is a protein with a molecular weight of about 29 kDa. It acts mechanically by aiding protein folding and assembly in the endoplasmic reticulum (ER). This protein is expressed in many tissues but more in the brain liver and pancreas. ERp29 contributes to cellular homeostasis by facilitating the proper folding of newly synthesized proteins within the ER.

Biological function summary

This protein assists in maintaining ER function by preventing the accumulation of misfolded proteins. ERp29 is part of the protein disulfide isomerase family which helps in forming disulfide bonds in proteins ensuring their correct structure and function. It does not form part of a larger complex but interacts with other proteins to manage stress and maintain cellular function. These interactions help in the secretory pathway important for processing proteins.

Pathways

ERp29 plays an innovative role in the Unfolded Protein Response (UPR) a cellular reaction to ER stress. It works alongside proteins like GRP78 within the UPR to mitigate stress's harmful effects by enhancing the protein folding capacity of the ER. Additionally ERp29's involvement in the protein disulfide isomerase family connects it to the oxidative protein folding pathway which is essential for maintaining redox balance in the ER.

ERp29's misregulation connects to neurodegenerative disorders such as Alzheimer's disease. It relates through its protein folding and stress response roles impacting cellular survival and function. Proteins like amyloid precursor protein (APP) show interaction with ERp29 in this context. Moreover in cancer biology ERp29 expression levels correlate with the progression of certain types of tumors as it influences pathways related to cell survival and apoptosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

See full target information Erp29

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102677 PubMed36336075

2022

A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yonatan G Mideksa,Isabel Aschenbrenner,Anja Fux,Dinah Kaylani,Caroline A M Weiß,Tuan-Anh Nguyen,Nina C Bach,Kathrin Lang,Stephan A Sieber,Matthias J Feige

PloS one 15:e0233502 PubMed32433667

2020

ERp29 as a regulator of Insulin biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Viviano,Margaret Brecker,Christine Ferrara-Cook,Laurence Suaud,Ronald C Rubenstein

The Journal of biological chemistry 294:18324-18336 PubMed31653700

2019

The KDEL receptor has a role in the biogenesis and trafficking of the epithelial sodium channel (ENaC).

Applications

Unspecified application

Species

Unspecified reactive species

Yann Bikard,Jeffrey Viviano,Melissa N Orr,Lauren Brown,Margaret Brecker,Jonathan Litvak Jeger,Daniel Grits,Laurence Suaud,Ronald C Rubenstein

Antioxidants (Basel, Switzerland) 8: PubMed31443506

2019

Limited Oxidative Stress Favors Resistance to Skeletal Muscle Atrophy in Hibernating Brown Bears ().

Applications

Unspecified application

Species

Unspecified reactive species

Blandine Chazarin,Anna Ziemianin,Alina L Evans,Emmanuelle Meugnier,Emmanuelle Loizon,Isabelle Chery,Jon M Arnemo,Jon E Swenson,Guillemette Gauquelin-Koch,Chantal Simon,Stéphane Blanc,Etienne Lefai,Fabrice Bertile

The Journal of biological chemistry 294:7177-7193 PubMed30877198

2019

The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec.

Applications

Unspecified application

Species

Unspecified reactive species

Rajaiah Pergu,Sunayana Dagar,Harsh Kumar,Rajesh Kumar,Jayanta Bhattacharya,Sivaram V S Mylavarapu

Oncology reports 41:1603-1615 PubMed30569094

2018

ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Guo,Lili Ma,Chao Liu,Yan Lei,Na Tang,Yingxin Huang,Guan Huang,Dazhou Li,Qi Wang,Guanglong Liu,Minshan Tang,Zhiliang Jing,Yongjian Deng

BioMed research international 2018:4374087 PubMed30643804

2018

Lutein and Zeaxanthin Isomers Reduce Photoreceptor Degeneration in the Mouse Model of Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Minzhong Yu,Weiming Yan,Craig Beight

Molecular medicine reports 13:2087-93 PubMed26781977

2016

Native low density lipoprotein promotes lipid raft formation in macrophages.

Applications

Unspecified application

Species

Mouse

Jian Song,Ling-Yan Ping,Duc M Duong,Xiao-Yan Gao,Chun-Yan He,Lei Wei,Jun-Zhu Wu

The Journal of biological chemistry 290:16824-40 PubMed25975270

2015

Loss of the Mono-ADP-ribosyltransferase, Tiparp, Increases Sensitivity to Dioxin-induced Steatohepatitis and Lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Shaimaa Ahmed,Debbie Bott,Alvin Gomez,Laura Tamblyn,Adil Rasheed,Tiffany Cho,Laura MacPherson,Kim S Sugamori,Yang Yang,Denis M Grant,Carolyn L Cummins,Jason Matthews

Molecular neurobiology 53:1874-1883 PubMed25790952

2015

Kainic Acid-Induced Golgi Complex Fragmentation/Dispersal Shifts the Proteolysis of Reelin in Primary Rat Neuronal Cells: An In Vitro Model of Early Stage Epilepsy.

Applications

ICC

Species

Unspecified reactive species

Yuji Kaneko,Robert Sullivan,Travis Dailey,Fernando L Vale,Naoki Tajiri,Cesar V Borlongan

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ERp29 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Western blot - Anti-ERp29 antibody (AB11420)

Western blot - Anti-ERp29 antibody (AB11420)

Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (AB11420)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise