Anti-ERp29 antibody [EPR12499(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ERp29 with ab176573 at 1 : 50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). Cells were fixed in 100% methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (2.5 µg/ml). DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing cytoplasmic staining in HepG2 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• WB

Lab

Western blot - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

Lanes 1-3 : Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ERP29 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ERP29 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-erp29-knockout-hek-293t-cell-line-ab266543'>ab266543</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Unknown

Western blot - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

Lanes 1-3 : Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ERP29 knockout HEK293T cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-ERp29 antibody [EPR12499(B)] (AB176573)

All lanes:

Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

OVCAR-3 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa

false