Rabbit Recombinant Monoclonal ERP29 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928), expandable thumbnail](https://content.abcam.com/products/images/erp29-antibody-epr12499b-bsa-and-azide-free-ab249928--western-blot-img6871.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928), expandable thumbnail](https://content.abcam.com/products/images/erp29-antibody-epr12499b-bsa-and-azide-free-ab249928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422701.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Expected | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Chicken | Not recommended | Not recommended | Predicted | Predicted | Predicted |
| Cow | Not recommended | Not recommended | Predicted | Predicted | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Target data
Function
Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Alternative names
C12orf8, ERP28, ERP29, Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 28, Endoplasmic reticulum resident protein 31, ERp28, ERp31
Recommended products
Rabbit Recombinant Monoclonal ERP29 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12499(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249928 is the carrier-free version of Anti-ERp29 antibody [EPR12499(B)] ab176573.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ERp29 also known as Endoplasmic Reticulum Resident Protein 29 is a protein with a molecular weight of about 29 kDa. It acts mechanically by aiding protein folding and assembly in the endoplasmic reticulum (ER). This protein is expressed in many tissues but more in the brain liver and pancreas. ERp29 contributes to cellular homeostasis by facilitating the proper folding of newly synthesized proteins within the ER.
- Biological function summary
This protein assists in maintaining ER function by preventing the accumulation of misfolded proteins. ERp29 is part of the protein disulfide isomerase family which helps in forming disulfide bonds in proteins ensuring their correct structure and function. It does not form part of a larger complex but interacts with other proteins to manage stress and maintain cellular function. These interactions help in the secretory pathway important for processing proteins.
- Pathways
ERp29 plays an innovative role in the Unfolded Protein Response (UPR) a cellular reaction to ER stress. It works alongside proteins like GRP78 within the UPR to mitigate stress's harmful effects by enhancing the protein folding capacity of the ER. Additionally ERp29's involvement in the protein disulfide isomerase family connects it to the oxidative protein folding pathway which is essential for maintaining redox balance in the ER.
- Associated diseases and disorders
ERp29's misregulation connects to neurodegenerative disorders such as Alzheimer's disease. It relates through its protein folding and stress response roles impacting cellular survival and function. Proteins like amyloid precursor protein (APP) show interaction with ERp29 in this context. Moreover in cancer biology ERp29 expression levels correlate with the progression of certain types of tumors as it influences pathways related to cell survival and apoptosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928), expandable thumbnail](https://content.abcam.com/products/images/erp29-antibody-epr12499b-bsa-and-azide-free-ab249928--western-blot-img6871.jpg "Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928)")
Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ERp29 antibody [EPR12499(B)] ab176573).
Lanes 1-3: Merged signal (red and green). Green - Anti-ERp29 antibody [EPR12499(B)] ab176573 observed at 29 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-ERp29 antibody [EPR12499(B)] ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ERP29 knockout HEK-293T cell line ab266543 (knockout cell lysate Human ERP29 knockout HEK-293T cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. Anti-ERp29 antibody [EPR12499(B)] ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ERp29 antibody [EPR12499(B)] (Anti-ERp29 antibody [EPR12499(B)] ab176573) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: ERP29 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human ERP29 knockout HEK-293T cell line (Human ERP29 knockout HEK-293T cell line ab266543)
Lane 3: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928), expandable thumbnail](https://content.abcam.com/products/images/erp29-antibody-epr12499b-bsa-and-azide-free-ab249928--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422701.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (ab249928)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ERp29 antibody [EPR12499(B)] ab176573).
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ERp29 with Anti-ERp29 antibody [EPR12499(B)] ab176573 at 1:50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). Cells were fixed in 100% methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (2.5 µg/ml). DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal image showing cytoplasmic staining in HepG2 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
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