AB249928

Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ERP29 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C12orf8, ERP28, ERP29, Endoplasmic reticulum resident protein 29, ERp29, Endoplasmic reticulum resident protein 28, Endoplasmic reticulum resident protein 31, ERp28, ERp31

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)

This data was developed using the same antibody clone in a different buffer formulation (ab176573). Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling ERp29 with ab176573 at 1 : 50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). Cells were fixed in 100% methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (2.5 µg/ml). DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing cytoplasmic staining in HepG2 cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Lab

Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)

This data was developed using the same antibody clone in a different buffer formulation (ab176573).

Lanes 1-3 : Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERp29 antibody [EPR12499(B)] (<a href='/en-us/products/primary-antibodies/erp29-antibody-epr12499b-ab176573'>ab176573</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ERP29 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ERP29 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-erp29-knockout-hek-293t-cell-line-ab266543'>ab266543</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Unconjugated

Anti-ERp29 antibody [EPR12499(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12499(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249928 is the carrier-free version of ab176573.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp29 also known as Endoplasmic Reticulum Resident Protein 29 is a protein with a molecular weight of about 29 kDa. It acts mechanically by aiding protein folding and assembly in the endoplasmic reticulum (ER). This protein is expressed in many tissues but more in the brain liver and pancreas. ERp29 contributes to cellular homeostasis by facilitating the proper folding of newly synthesized proteins within the ER.

Biological function summary

This protein assists in maintaining ER function by preventing the accumulation of misfolded proteins. ERp29 is part of the protein disulfide isomerase family which helps in forming disulfide bonds in proteins ensuring their correct structure and function. It does not form part of a larger complex but interacts with other proteins to manage stress and maintain cellular function. These interactions help in the secretory pathway important for processing proteins.

Pathways

ERp29 plays an innovative role in the Unfolded Protein Response (UPR) a cellular reaction to ER stress. It works alongside proteins like GRP78 within the UPR to mitigate stress's harmful effects by enhancing the protein folding capacity of the ER. Additionally ERp29's involvement in the protein disulfide isomerase family connects it to the oxidative protein folding pathway which is essential for maintaining redox balance in the ER.

ERp29's misregulation connects to neurodegenerative disorders such as Alzheimer's disease. It relates through its protein folding and stress response roles impacting cellular survival and function. Proteins like amyloid precursor protein (APP) show interaction with ERp29 in this context. Moreover in cancer biology ERp29 expression levels correlate with the progression of certain types of tumors as it influences pathways related to cell survival and apoptosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

See full target information ERP29

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)

Western blot - Anti-ERp29 antibody [EPR12499(B)] - BSA and Azide free (AB249928)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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