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AB192444

Anti-ERp57 antibody - C-terminal

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(1 Publication)

Goat Polyclonal ERp57 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA3 aa 300 to C-terminus.

View Alternative Names

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody - C-terminal (AB192444)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody - C-terminal (AB192444)

Immunofluorescent analysis of RPE cells (4% PFA-fixed) labeling ERp57 with ab192444 at 1/100 dilution.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDIA3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30101

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Alternative Products

Primary Antibodies

AB10287

Anti-ERp57 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

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Primary Antibodies

AB13506

Anti-ERp57 antibody [MaP.Erp57]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [MaP.Erp57] (AB13506)

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Primary Antibodies

AB154191

Anti-ERp57 antibody [EPR10678(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (AB154191)

  • 0
  • 0
Primary Antibodies

AB154197

Anti-ERp57 antibody [EPR10679(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (AB154197)

  • 0
  • 0
Primary Antibodies

AB183396

FITC Anti-ERp57 antibody [Map.ERP57]

  • 0
  • 0

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

AB8227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.
Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).
See full target information PDIA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:698233 PubMed36176271

2022

TAK1 inhibition increases proliferation and differentiation of chick retinal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Casandra Carrillo,Vagisha Ravi,Sarika Tiwari,Ellen A Chernoff,Teri L Belecky-Adams
View all publications
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Product promise

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