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AB242367

Anti-ERp57 antibody [CL2444]

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(1 Publication)

Mouse Monoclonal ERp57 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA3 aa 100-250.

View Alternative Names

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

11 Images
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human cerebral cortex tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of A431 (human epidermoid carcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of U-251 MG (Human brain glioma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Negative control :

Paraffin-embedded human skeletal muscle tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human thyroid gland tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human endometrium tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)
  • WB

Supplier Data

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)

All lanes:

Western blot - Anti-ERp57 antibody [CL2444] (ab242367) at 1 µg/mL

Lane 1:

U-251 MG (Human brain glioma cell line) cells transfected with a control siRNA

Lane 2:

U-251 MG cells transfected with a target-specific siRNA probe

Lane 3:

U-251 MG cells transfected with a second target-specific siRNA probe

Predicted band size: 57 kDa

false

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)
  • WB

Supplier Data

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)

All lanes:

Western blot - Anti-ERp57 antibody [CL2444] (ab242367)

Predicted band size: 57 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2444

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDIA3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P30101

Epitope

Binds to an epitope located within the peptide sequence KKFISDKDASIVGFF as determined by overlapping synthetic peptides.

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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  • 0
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Primary Antibodies

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Secondary Antibodies

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Sample Prep & Detection Kits

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Schematic Diagram - Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (AB269822)

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Sample Prep & Detection Kits

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Schematic Diagram - DyLight® 755 Conjugation Kit (Fast) - Lightning-Link® (AB201805)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.
Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).
See full target information PDIA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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