AB155800

Anti-ERp72 antibody

Name: Anti-ERp72 antibody (ab155800) | Abcam
Brand: Abcam Limited
SKU: AB155800
Price: 535 USD
Availability: InStock

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(15 Publications)

Rabbit Polyclonal ERp72 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA4 aa 350 to C-terminus.

View Alternative Names

ERP70, ERP72, PDIA4, Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ER protein 72, ERp-72, ERp72

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (AB155800)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (AB155800)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling ERp72 with ab155800 at 1/500 dilution (green). Bottom image shows merger with nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (AB155800)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (AB155800)

Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling ERp72with ab155800 at 1/500 dilution.

Unknown

Western blot - Anti-ERp72 antibody (AB155800)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ERp72 antibody (ab155800) at 1/10000 dilution

Lane 1:

NIH-3T3 whole cell lysate at 30 µg

Lane 2:

JC whole cell lysate at 30 µg

Lane 3:

BCL-1 whole cell lysate at 30 µg

Predicted band size: 73 kDa

false

Unknown

Western blot - Anti-ERp72 antibody (AB155800)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ERp72 antibody (ab155800) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 73 kDa

false

Supplier Data

Western blot - Anti-ERp72 antibody (AB155800)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-ERp72 antibody (ab155800) at 1/1000 dilution

Lane 1:

Wild-type ERP72 293T cell extracts at 30 µg

Lane 2:

Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PDIA4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13667

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp72 also known as PDIA4 is a protein with a molecular weight of approximately 72 kDa. It belongs to the protein disulfide isomerase (PDI) family and functions as an enzyme within the endoplasmic reticulum (ER). The protein helps with folding by catalyzing the formation rearrangement and breakage of disulfide bonds in nascent proteins. ERp72 is expressed in a variety of tissues with high abundance in the liver pancreas and other secretory organs reflecting its role in protein synthesis and processing.

Biological function summary

The protein disulfide isomerase family member ERp72 plays a significant part in maintaining ER function. It participates in the unfolded protein response (UPR) which ensures proper protein folding and manages ER stress. ERp72 operates as part of a larger multiprotein complex that aids in the quality control of newly synthesized proteins. The protein interacts with calnexin and BiP to perform these functions. This makes ERp72 essential for cellular homeostasis and adaptation to changing physiological conditions.

Pathways

ERp72 is closely involved in the protein folding and ER stress response pathways. The unfolded protein response pathway recognizes misfolded proteins in the ER and initiates mechanisms to restore normal function. It connects with other proteins such as ATF6 and IRE1 to modulate gene expression and improve protein folding capacity. Additionally ERp72 participates in the pathway related to oxidative protein folding working alongside proteins like ERO1. This confirms its key contribution to cellular function by maintaining the balance between protein folding and degradation.

Alterations in ERp72 expression or function link to conditions such as neurodegenerative diseases and cancer. Alzheimer's disease shows aberrant ER stress and misfolded protein accumulation where ERp72's interaction with amyloid precursor protein might play a role. In the case of cancer ER stress and UPR pathway alterations contribute to cancer cell survival associating ERp72 with this adaptation process particularly its interaction with BiP and other PDIs. These connections highlight the relevance of ERp72 in disease progression and as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PDIA4

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7699 PubMed40825974

2025

Noncanonical function of Pannexin1 promotes cellular senescence and renal fibrosis post-acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Liuwei Huang,Yanting Shen,Xiaoling Pan,Jiaqi Li,Caizhen Li,Lixin Ruan,Sitan He,Lanlan Huang,Kangyi Liu,Xin Zhao,Jian Geng,Jie Guo,Fan Fan Hou,Jun Wang

Nature communications 16:2535 PubMed40087276

2025

ER O-glycosylation in synovial fibroblasts drives cartilage degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Son Tran,Joanne Chia,Xavier Le Guezennec,Keit Min Tham,Anh Tuan Nguyen,Virginie Sandrin,Way Cherng Chen,Tan Tong Leng,Sreedharan Sechachalam,Khai Pang Leong,Frederic A Bard

Cell reports. Medicine 6:101917 PubMed39809267

2025

Spatial covariance reveals isothiocyanate natural products adjust redox stress to restore function in alpha-1-antitrypsin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Sun,Chao Wang,Junyan Hu,Pei Zhao,Xi Wang,William E Balch

The breast journal 2023:3970444 PubMed37720188

2023

LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17.

Applications

Unspecified application

Species

Unspecified reactive species

Chanjiao Yao,Lingjuan Zeng,Qin Liu,Xiaoxin Qiu,Chunyan Chen

iScience 26:106328 PubMed36968068

2023

Mitochondria-associated membrane protein PACS2 maintains right cardiac function in hypobaric hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Mengjia Sun,Renzheng Chen,Xiaowei Ye,Boji Wu,Zhen Liu,Jihang Zhang,Xubin Gao,Ran Cheng,Chunyan He,Jingyu He,Xuhong Wang,Lan Huang

Cell chemical biology 30:22-42.e5 PubMed36630963

2023

Capturing the conversion of the pathogenic alpha-1-antitrypsin fold by ATF6 enhanced proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhong Sun,Chao Wang,Pei Zhao,Gabe M Kline,Julia M D Grandjean,Xin Jiang,Richard Labaudiniere,R Luke Wiseman,Jeffery W Kelly,William E Balch

The EMBO journal 42:e111869 PubMed36245281

2022

The disulfide catalyst QSOX1 maintains the colon mucosal barrier by regulating Golgi glycosyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Tal Ilani,Nava Reznik,Noa Yeshaya,Tal Feldman,Patrick Vilela,Zipora Lansky,Gabriel Javitt,Michal Shemesh,Ori Brenner,Yoav Elkis,Neta Varsano,Ana M Jaramillo,Christopher M Evans,Deborah Fass

International journal of molecular sciences 22: PubMed34884824

2021

Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney L Lane,Jason C Parks,Jennifer E Russ,Shaihla A Khan,William B Schoolcraft,Ye Yuan,Mandy G Katz-Jaffe

eLife 10: PubMed34190687

2021

A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process.

Applications

Unspecified application

Species

Unspecified reactive species

Jimena Pérez-Vargas,Elin Teppa,Fouzia Amirache,Bertrand Boson,Rémi Pereira de Oliveira,Christophe Combet,Anja Böckmann,Floriane Fusil,Natalia Freitas,Alessandra Carbone,François-Loïc Cosset

Immunity, inflammation and disease 9:108-119 PubMed33159506

2020

MicroRNA-378a-3p contributes to ovarian cancer progression through downregulating PDIA4.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Chanjiao,Chen Chunyan,Qiu Xiaoxin,Han Youjian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp72 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp72 antibody (AB155800)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody (AB155800)

Western blot - Anti-ERp72 antibody (AB155800)

Western blot - Anti-ERp72 antibody (AB155800)

Western blot - Anti-ERp72 antibody (AB155800)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise