AB190348

Anti-ERp72 antibody [EPR14824(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal ERp72 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ERP70, ERP72, PDIA4, Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ER protein 72, ERp-72, ERp72

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ERp72 with ab190348 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde-fixed MCF7 cells labeling ERp72 with ab190348 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Supplier Data

Western blot - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

All lanes:

Western blot - Anti-ERp72 antibody [EPR14824(B)] (ab190348) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

JAR cell lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

true

Carrier free

Anti-ERp72 antibody [EPR14824(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14824(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERp72 also known as PDIA4 is a protein with a molecular weight of approximately 72 kDa. It belongs to the protein disulfide isomerase (PDI) family and functions as an enzyme within the endoplasmic reticulum (ER). The protein helps with folding by catalyzing the formation rearrangement and breakage of disulfide bonds in nascent proteins. ERp72 is expressed in a variety of tissues with high abundance in the liver pancreas and other secretory organs reflecting its role in protein synthesis and processing.

Biological function summary

The protein disulfide isomerase family member ERp72 plays a significant part in maintaining ER function. It participates in the unfolded protein response (UPR) which ensures proper protein folding and manages ER stress. ERp72 operates as part of a larger multiprotein complex that aids in the quality control of newly synthesized proteins. The protein interacts with calnexin and BiP to perform these functions. This makes ERp72 essential for cellular homeostasis and adaptation to changing physiological conditions.

Pathways

ERp72 is closely involved in the protein folding and ER stress response pathways. The unfolded protein response pathway recognizes misfolded proteins in the ER and initiates mechanisms to restore normal function. It connects with other proteins such as ATF6 and IRE1 to modulate gene expression and improve protein folding capacity. Additionally ERp72 participates in the pathway related to oxidative protein folding working alongside proteins like ERO1. This confirms its key contribution to cellular function by maintaining the balance between protein folding and degradation.

Alterations in ERp72 expression or function link to conditions such as neurodegenerative diseases and cancer. Alzheimer's disease shows aberrant ER stress and misfolded protein accumulation where ERp72's interaction with amyloid precursor protein might play a role. In the case of cancer ER stress and UPR pathway alterations contribute to cancer cell survival associating ERp72 with this adaptation process particularly its interaction with BiP and other PDIs. These connections highlight the relevance of ERp72 in disease progression and as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PDIA4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:747-751 PubMed38467876

2024

Structural basis of human U5 snRNP late biogenesis and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Riabov Bassat,Supapat Visanpattanasin,Matthias K Vorländer,Laura Fin,Alexander W Phillips,Clemens Plaschka

Aging 15:15578-15598 PubMed38159261

2024

Development of a prognostic model for glioblastoma multiforme based on the expression levels of efferocytosis-related genes.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhe Xu,Lihui Han,Pengfei Zhu,Yufeng Cheng,Xuan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp72 antibody [EPR14824(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Flow Cytometry (Intracellular) - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Western blot - Anti-ERp72 antibody [EPR14824(B)] (AB190348)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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