AB131211

Anti-ESD antibody [EPR8446]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ESD antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

3 Images

Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] (AB131211)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] (AB131211)

ab131211 at 1/10 labelling ESD in permeabilized Jurkat cells by intracellular flow cytometry. Rabbit IgG negative control is in green.

Lab

Western blot - Anti-ESD antibody [EPR8446] (AB131211)

Lanes 1-4 : Merged signal (red and green). Green - ab131211 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.

ab131211 Anti-ESD antibody [EPR8446] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab131211 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ESD knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-esd-knockout-hek-293t-cell-lysate-ab257942'>ab257942</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

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Unknown

Western blot - Anti-ESD antibody [EPR8446] (AB131211)

All lanes:

Western blot - Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 31 kDa

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Carrier free

Anti-ESD antibody [EPR8446] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8446

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ESD also known as esterase D or S-formylglutathione hydrolase is a protein with a mass of approximately 31 kDa. ESD is expressed in various tissues and cells including HEK-293T cells. Cell lysate studies often utilize the ESD line to analyze its enzymatic activity and expression profile. The protein plays a mechanical role in hydrolyzing esters of glutathione contributing to the detoxification processes within the cell.

Biological function summary

ESD contributes to maintaining cellular homeostasis through its role in detoxification and response to oxidative stress. It interacts with the degradation of S-formylglutathione a byproduct formed during alcohol metabolism. ESD is not typically associated with a larger protein complex but its enzymatic function is important for cellular health and efficient elimination of toxic metabolites protecting cells from damage and supporting metabolic pathways.

Pathways

ESD's enzymatic activity is integrated into the glutathione metabolism and alcohol metabolism pathways. Here ESD acts alongside enzymes like glutathione S-transferase to convert harmful aldehydes into less toxic forms aiding in cellular resilience. The protein helps modulate oxidative stress responses cooperating indirectly with other detoxification proteins within these pathways to maintain optimal cellular function.

ESD associations include certain forms of cancer and neurodegenerative diseases. Abnormal ESD expression or activity can result in disturbed detoxification leading to oxidative stress and cell damage. In cancer ESD has been implicated in tumor progression potentially linked with other proteins like GSTP1 which also participates in detoxification. In neurodegenerative conditions defective detoxification pathways involving ESD may contribute to neuronal injury and disease progression making it a potential target for therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine hydrolase involved in the detoxification of formaldehyde.

See full target information S-formylglutathione hydrolase

Alternative Names

S-formylglutathione hydrolase, FGH, Esterase D, Methylumbelliferyl-acetate deacetylase, ESD

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 8:183 PubMed29900129

2018

Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Bhupesh K Prusty,Suvagata R Chowdhury,Nitish Gulve,Thomas Rudel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ESD antibody [EPR8446]

Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] (AB131211)

Western blot - Anti-ESD antibody [EPR8446] (AB131211)

Western blot - Anti-ESD antibody [EPR8446] (AB131211)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise