Rabbit Recombinant Monoclonal ESD antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--western-blot-img2396.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372)")
![Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--flow-cytometry-intracellular-img57439.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372)")
![Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--western-blot-img116555.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (AB248372)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Serine hydrolase involved in the detoxification of formaldehyde.
Alternative names
S-formylglutathione hydrolase, FGH, Esterase D, Methylumbelliferyl-acetate deacetylase, ESD
Recommended products
Rabbit Recombinant Monoclonal ESD antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8446
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248372 is the carrier-free version of Anti-ESD antibody [EPR8446] ab131211.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ESD also known as esterase D or S-formylglutathione hydrolase is a protein with a mass of approximately 31 kDa. ESD is expressed in various tissues and cells including HEK-293T cells. Cell lysate studies often utilize the ESD line to analyze its enzymatic activity and expression profile. The protein plays a mechanical role in hydrolyzing esters of glutathione contributing to the detoxification processes within the cell.
- Biological function summary
ESD contributes to maintaining cellular homeostasis through its role in detoxification and response to oxidative stress. It interacts with the degradation of S-formylglutathione a byproduct formed during alcohol metabolism. ESD is not typically associated with a larger protein complex but its enzymatic function is important for cellular health and efficient elimination of toxic metabolites protecting cells from damage and supporting metabolic pathways.
- Pathways
ESD's enzymatic activity is integrated into the glutathione metabolism and alcohol metabolism pathways. Here ESD acts alongside enzymes like glutathione S-transferase to convert harmful aldehydes into less toxic forms aiding in cellular resilience. The protein helps modulate oxidative stress responses cooperating indirectly with other detoxification proteins within these pathways to maintain optimal cellular function.
- Associated diseases and disorders
ESD associations include certain forms of cancer and neurodegenerative diseases. Abnormal ESD expression or activity can result in disturbed detoxification leading to oxidative stress and cell damage. In cancer ESD has been implicated in tumor progression potentially linked with other proteins like GSTP1 which also participates in detoxification. In neurodegenerative conditions defective detoxification pathways involving ESD may contribute to neuronal injury and disease progression making it a potential target for therapeutic exploration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--western-blot-img2396.jpg "Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)")
Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
This data was developed using Anti-ESD antibody [EPR8446] ab131211, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - Anti-ESD antibody [EPR8446] ab131211 observed at 31 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-ESD antibody [EPR8446] ab131211 Anti-ESD antibody [EPR8446] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human ESD knockout HEK-293T cell line ab266360 (knockout cell lysate Human ESD knockout HEK-293T cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. Anti-ESD antibody [EPR8446] ab131211 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ESD antibody [EPR8446] (Anti-ESD antibody [EPR8446] ab131211) at 1/1000 dilution
Lane 1: Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: Western blot - Human ESD knockout HEK-293T cell line (Human ESD knockout HEK-293T cell line ab266360)
Lane 3: K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
![Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--flow-cytometry-intracellular-img57439.jpg "Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)")
Flow Cytometry (Intracellular) - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
This data was developed using Anti-ESD antibody [EPR8446] ab131211, the same antibody clone in a different buffer formulation.
Anti-ESD antibody [EPR8446] ab131211 at 1/10 labelling ESD in permeabilized Jurkat cells by intracellular flow cytometry. Rabbit IgG negative control is in green.
![Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372), expandable thumbnail](https://content.abcam.com/products/images/esd-antibody-epr8446-bsa-and-azide-free-ab248372--western-blot-img116555.jpg "Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)")
Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
This data was developed using Anti-ESD antibody [EPR8446] ab131211, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ESD antibody [EPR8446] (Anti-ESD antibody [EPR8446] ab131211) at 1/1000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: Caco-2 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Predicted band size: 31 kDa
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