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AB254774

Anti-Espin antibody

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(1 Publication)

Rabbit Polyclonal Espin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ESPN aa 750-850.

View Alternative Names

DFNB36, LP2654, ESPN, Espin, Autosomal recessive deafness type 36 protein, Ectoplasmic specialization protein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)

Immunohistochemical analysis of human kidney tissue labeling Espin with ab254774 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)

Immunohistochemical analysis of human skeletal muscle tissue labeling Espin with ab254774 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)

Immunohistochemical analysis of human testis tissue labeling Espin with ab254774 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Espin antibody (AB254774)

Immunohistochemical analysis of human duodenum tissue labeling Espin with ab254774 at a 1/1000 dilution.

Western blot - Anti-Espin antibody (AB254774)
  • WB

Supplier Data

Western blot - Anti-Espin antibody (AB254774)

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Western blot - Anti-Espin antibody (ab254774)

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Western blot - Anti-Espin antibody (ab254774) at 0.4 µg/mL

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MW Ladder

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SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ESPN aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

B1AK53

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Espin also known as actin-bundling protein is a protein important for the development of hair cell stereocilia in cochlea. It has a molecular mass of approximately 116 kDa. Espin is mainly expressed in cells that have hair-like projections which include hair cells of the inner ear and in the Sertoli cells in testis. These cells rely on Espin to maintain their structure and function playing a pivotal role in stabilizing the parallel actin filament bundles essential for cell projections' rigidity and strength.
Biological function summary

Espin is essential for actin cytoskeleton organization. It acts as an important component in forming cross-links between actin filaments enhancing their stability and organization. Espin is not generally considered part of a complex but it directly interacts with actin in cells. The protein influences cellular processes like cell shape alterations and sensory cell function by regulating actin assembly dynamics. This ability to control actin filaments directly impacts how sensory inputs like sound vibration are relayed and processed.

Pathways

Espin plays a substantial role in the actin cytoskeleton reorganization pathway which is integral to maintaining cellular architecture and mechanotransduction. Espin associates with other actin-binding proteins such as fascin and fimbrin which also contribute to actin bundling and stabilization. Additionally Espin's interactions with these proteins support stereocilia formation and maintenance critical for auditory and balance functions in mammals.

Espin mutations have shown connections to disorders like Non-syndromic Deafness and Usher Syndrome. In Non-syndromic Deafness Espin dysfunction can lead to impaired stereocilia development resulting in hearing loss. Similarly mutations affecting Espin are linked with Usher Syndrome where hearing and vision are disrupted often with Espin's interactions with protocadherin proteins being implicated. Understanding these associations highlights the importance of Espin's proper function in sensory health and stress the potential impact of its dysregulation in related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional actin-bundling protein. Plays a major role in regulating the organization, dimension, dynamics and signaling capacities of the actin filament-rich microvilli in the mechanosensory and chemosensory cells (PubMed : 29572253). Required for the assembly and stabilization of the stereociliary parallel actin bundles. Plays a crucial role in the formation and maintenance of inner ear hair cell stereocilia (By similarity). Involved in the elongation of actin in stereocilia (PubMed : 29572253). In extrastriolar hair cells, required for targeting MYO3B to stereocilia tips, and for regulation of stereocilia diameter and staircase formation.
See full target information ESPN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 78:100129 PubMed36473368

2022

Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2.

Applications

Unspecified application

Species

Unspecified reactive species

Binya Hu,Min Huang,Lihua Tao,Yun Li,Yuting Kuang,Guangliang Liu,Sijun Zhao
View all publications
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