AB155227

Anti-ESRP2 antibody

Name: Anti-ESRP2 antibody (ab155227) | Abcam
Brand: Abcam Limited
SKU: AB155227

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(3 Publications)

Rabbit Polyclonal ESRP2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ESRP2.

View Alternative Names

RBM35B, PP7059, ESRP2, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B, RNA-binding protein 35B

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ESRP2 antibody (AB155227)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ESRP2 antibody (AB155227)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling ESRP2 (green) using ab155227 at a 1/500 dilution. The slide was costained with an anti-alpha Tubulin (red).

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Western blot - Anti-ESRP2 antibody (AB155227)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ESRP2 antibody (ab155227) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 44 kDa,78 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ESRP2.

Q9H6T0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ESRP2 also known as Epithelial Splicing Regulatory Protein 2 plays a role in how RNA undergoes alternative splicing. This protein is part of the larger family of RNA-binding proteins that modulate the splicing of pre-mRNA in an epithelial cell context. ESRP2 has a molecular weight of approximately 76 kDa. It is expressed mainly in epithelial tissues including the lung kidney and intestine. Human ESRP2 encodes a protein that controls variations in mRNA which leads to different protein isoforms.

Biological function summary

ESRP2 regulates epithelial cell phenotypes by influencing the expression of genes specific to epithelial tissues. It forms a complex with ESRP1 another RNA-binding protein enhancing its ability to control RNA splicing events. The ESRP2 protein affects the alternative splicing of pre-mRNAs encoding proteins that contribute to cell adhesion motility and signaling pathways. This precise regulation of mRNA variants is critical for maintaining epithelial cellular identity and function.

Pathways

The involvement of ESRP2 in RNA splicing impacts the epithelial-mesenchymal transition (EMT) pathway. This is key in developmental processes and cancer metastasis where ESRP2 partners with ESRP1 to regulate the splice variants of genes important for EMT like FGFR2. ESRP2 affects the inclusion or exclusion of certain exons driving variation in receptor forms that have different biological outcomes influencing cell signaling and adhesion properties.

ESRP2 has significant associations with cancer progression particularly in epithelial tumors such as breast cancer and prostate cancer. Abnormal ESRP2 expression can lead to changes in EMT-related gene splicing fostering metastasis and advanced tumor states. In the context of prostate cancer ESRP2 interacts with FGFR2 and influences cancer cell invasiveness by altering specific splicing patterns highlighting its potential as a therapeutic target in cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

See full target information ESRP2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular neurobiology 43:1199-1218 PubMed35748966

2022

LncRNA Pnky Positively Regulates Neural Stem Cell Migration by Modulating mRNA Splicing and Export of Target Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiannan Du,Yuan Li,Yuting Su,Wenqian Zhi,Jiale Zhang,Cheng Zhang,Juan Wang,Wensheng Deng,Shasha Zhao

Molecular oncology 16:630-647 PubMed34520622

2021

The epithelial splicing regulator ESRP2 is epigenetically repressed by DNA hypermethylation in Wilms tumour and acts as a tumour suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Danny Legge,Ling Li,Whei Moriarty,David Lee,Marianna Szemes,Asef Zahed,Leonidas Panousopoulos,Wan Yun Chung,Yara Aghabi,Jasmin Barratt,Richard Williams,Kathy Pritchard-Jones,Karim T A Malik,Sebastian Oltean,Keith W Brown

Oncotarget 12:626-637 PubMed33868584

2021

Inhibition of resistant triple-negative breast cancer cells with low-dose 6-mercaptopurine and 5-azacitidine.

Applications

Unspecified application

Species

Unspecified reactive species

Balraj Singh,Vanessa N Sarli,Anthony Lucci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ESRP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ESRP2 antibody (AB155227)

Western blot - Anti-ESRP2 antibody (AB155227)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise