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AB221960

Anti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Estriol antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

E3, Oestriol

2 Images
Competitive ELISA - Anti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free (AB221960)
  • cELISA

Unknown

Competitive ELISA - Anti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free (AB221960)

This ELISA data was generated with the same anti-Estriol antibody clone [EPPTXR3] in a different buffer formulation (cat# ab150407).

2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1%BSA serial dilution of Estriol (0 0.1 0.5 2 5 10 20 and 50ng/ml) were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96-wells. After washing TMB substrate was used to develop the color.

Competitive ELISA - Anti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free (AB221960)
  • cELISA

Unknown

Competitive ELISA - Anti-Estriol antibody [EPPTXR3] - Low endotoxin, Azide free (AB221960)

This ELISA data was generated using the same anti-Estriol antibody clone [EPPTXR3] in a different buffer formulation (cat# ab150407).

2 ug/ml of goat anti-rabbit IgG was coated into 96-wells. After blocking with 1% BSA 20ng/ml of various buffer containing Estriol Estradiol Estrone 16-Epiestriol Estriol 3-sulfate 16-Hydroxyestrone or PBS buffer only were added together with HRP conjugated Estriol (1/100) and 0.075 ug/ml of ab150407 into 96- wells. After washing TMB substrate was used to develop the color.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTXR3

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab221960 is the carrier-free version of ab150407.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Estriol sometimes referred to simply as E3 possesses a molecular mass of 288.38 g/mol. This estrogenic hormone is one of three major estrogens in humans alongside estrone and estradiol. It is produced in significant amounts during pregnancy primarily by the placenta and circulates in the blood of pregnant women. Estriol can also be detected albeit in much lower levels in non-pregnant individuals and is synthesized in the liver from 16-hydroxydehydroepiandrosterone sulfate. Due to its significant role in pregnancy measuring estriol levels can provide valuable information about fetal health and development.
Biological function summary

Estriol plays a pivotal role in the maturation and function of female reproductive organs and maintains the uterus during pregnancy. Its function involves the modulation of the expression of genes associated with reproductive tissues. Estriol does not generally form part of a complex operating instead as a free hormone that exerts effects by binding directly to estrogen receptors located in various tissues. Despite being considered a weaker estrogen compared to estradiol estriol is essential in the biological processes it governs particularly due to its presence and function during pregnancy.

Pathways

Estriol participates in the estrogen signaling pathway which is important for regulating the reproductive system and secondary sexual characteristics. It interacts with estrogen receptors ERα and ERβ which lead to gene transcription and subsequent biological effects. Furthermore estriol is involved in hormone synthesis pathways intersecting with the biosynthesis of other steroid hormones. Related proteins such as aromatase play a significant role in these pathways by converting androgens into estrogens including estriol thereby influencing its levels and activity.

Aberrant estriol levels associate with certain conditions. Low estriol particularly during pregnancy may indicate fetal distress or chromosomal abnormalities such as Down syndrome. Additionally its role in hormone-related cancers particularly breast and endometrial cancer has been studied though more research is required to fully understand these associations. Estriol's interactions with proteins such as the estrogen receptors ERα and ERβ suggest potential mechanisms through which these disorders might arise highlighting the importance of further investigation into estriol's broader biological impact.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Estriol
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular cell biology 11:636-648 PubMed30690467

2019

Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Peng,Ai-Li Sheng,Qi Xiao,Libing Shen,Xiang-Chun Ju,Min Zhang,Si-Ting He,Chao Wu,Zhen-Ge Luo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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